Quaternary Ammonium Chitosan Nanoparticles Cross-linked with Genipin and Sodium Tripolyphosphate as Drug Delivery Carriers

• Main Authors: ZENG, SI-YING, 曾思盈
• Other Authors: CHEN, KUO-YU
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/8v8vn4

碩士 === 國立雲林科技大學 === 化學工程與材料工程系 === 106 === Biopolymers are widely used as drug delivery carries to improve the utility of drugs and reduce toxic side effects. Chitosan is used as drug delivery systems since it is non-toxic, biocompatible, biodegradable mucoadhesive polymer. However, its limited solubility. Thus, the application of chitosan only in acidic condition but not in the intestinal tract where the pH values are higher than 6.5. To overcome this problem, introducing quaternary ammonium group on the chitosan backbone. Quaternary ammonium chitosan (QCS) with improved solubility and enhanced positive charge intensity. In this study, QCS with different degrees of grafting yield was synthesized by graft polymerization and characterized by NMR, FTIR and XRD measurements. The aim of this study is to optimize the preparation of bovine serum albumin (BSA) loaded QCS nanoparticles by ionic/chemical co-crosslinking method using Box-Behnken design. The independent variables (grafting yield (%), QCS:TPP mass ratio and QCS:Genipin mass ratio) were taken to investigate their effect on dependent variables (particle size, polydispersity index (PDI), zeta potential, entrapment efficiency (EE%)). The formulation variables were optimized to achieve particle size < 200 nm, PDI < 0.3 and maximum entrapment efficiency. The optimized nanoparticles formulation was characterized for particle size, PDI, zeta potential, EE% and in vitro drug release. Results for particle size, PDI, zeta potential, EE% were found to be 193.68 ± 44.92 nm, 0.232, 23.97 mV, 46.37 ± 2.89 %, respectively. In vitro release studies showed a sustained release from genipin crosslinking QCS nanoparticles as compared to QCS nanoparticles. The results indicate that genipin crosslinking QCS nanoparticles may be as promising protein drug carriers for a sustained delivery.