Developed an Elastic Scaffold by Poly(glycerol sebacate) and Ectoine Copolymer for Vascular Tissue Engineering

• Main Authors: Chun-Hui Li, 李軍慧
• Other Authors: Chao-Ling Yao
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/v3r4ff

碩士 === 元智大學 === 化學工程與材料科學學系 === 106 === Currently, repair and regeneration of tissue by engineered vascular tissue is an important research topic for biomedical engineering and regenerative medicine. Many studies indicated that an excellent scaffold for tissue engineering should have the good abilities to let cells attach and to let vessel grow in it. Our previous study showed that poly (glycerol sebacate) (PGS100) is an elastomeric biodegradable polyester, and has good biocompatibility for vessel tissue engineering. In view of the potential use of PGS100 in tissue engineering, the incorporation of Ectoine into PGS100 can enhance the hydrophilic and biocompatible properties of PGS100. Therefore, in this study, we attempted to characterize the PGS polymer by condensation polymerization reaction and to mix PGS100 with different ratios of Ectoine (molar ratios are 1.4, 4.2, 7.0, 10.5, 14.0) to enhance the protein expression and to stabilize the cell structure. Our results showed the greater the amount of Ectoine added, the greater the hydrophilicity of the material. ATR-FTIR was used to measure the change of functional groups before and after mixed with different ratio of Ectoine. Our data showed that with the increase of the amount of Ectoine, there were more obvious N-H bond at 1400-1600 cm-1 absorption peak. TGA analysis showed that the thermal cracking at 280°C. XPS elemental composition ratio analysis indicated that the more Ectoine added, the higher N / C ratio. Taken above data together, the results demonstrated that the PGS100 has been successfully mixed with Ectoine in the surface and in the inside of material. Measurement of degree of crosslink after synthesis different ratio of PGS100 biopolymer elastomeric copolymer, which showed that added more ratio of Ectoine cause the degree of crosslink decreased. The tensile test showed that added more ratio of Ectoine cause the elongation increased. Otherwise, the mass residual rate of PGS100/E14.0 was remained 74.7% after 13-week degradation test. The above results showed that the PGS100/E elastomeric scaffold is a good biodegradable material. Finally, the results showed that L929 fibroblast cells and endothelial progenitor cell (EPCs) can attach and proliferate on the PGS100/E without significant cytotoxic effect. Overall, PGS100/E is a biocompatible and biodegradable material, and it is suitable for the growth of EPCs. In the future, we believe that PGS100/E will show a wide application potential in vascular tissue engineering.