Design of Biosensor and Development of Its Detection Platform

• Main Authors: YANG, CHU-CHUN, 楊筑鈞
• Other Authors: LIN, YUE-DER
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/7q2cnf

碩士 === 逢甲大學 === 生醫資訊暨生醫工程碩士學位學程 === 107 === The detection of specific viruses or proteins is a necessary means to prevent or diagnose certain diseases. In order to achieve the purpose of point of care, the development of biosensors is an indispensable key to this trend. However, in a medically developed world, the average age is getting higher and higher, leading to the current social promotion of long-term care, home care and other policies, so the use of detection instruments is also becoming more general. Taiwan's semiconductor industry has a global competitive advantage, and the nanowire field effect transistor biosensor has the advantages of high stability and high sensitivity. The nanowire sensing components of this paper were used a self-programming process to commissioned National Nano Device Laboratories, but most of these components were made of semiconductor materials such as Silicon or Germanium, and the cost was relatively high. Therefore, this paper also uses an interdigital electrode to make a biosensor with the advantages of high accuracy and high sensitivity. In order to make the detection instrument universal, this thesis develops the circuit design method of the interdigitated electrode biosensor and its detection platform, which simplifies the use method, can be measured without using expensive instruments, and even has no knowledge of medical instruments. Users are able to operate and used in a variety of different test situations with universal and portable features to meet the needs of point-of-care, long-term care and home care. This thesis cooperates with Professor Yit Lung Khung from the China Medical University Department of Biological Science and Technology, used the nanowire field-effect transistor fabricated by the self-programming process. In the laboratory of Professor Yit Lung Khung of China Medical University, used silane to make chemical modification on the silicon nanowire, and using the Thiol method to chemically modify the gold on the surface of the interdigitated electrode, and the DNA used vascular endothelial growth factor to detect the protein. In addition, in order to make the sensing component achieve the goal of point-of-care, long-term care and home medical treatment, this paper will also design this simple detection platform, with a high-order 32-bit microcontroller to transmit the results by universal asynchronous transmission, and show the results on the graphical user interface. The results of this paper can be applied to research, medical treatment, and can also be applied to daily life, such as pesticide testing, impurity oil testing or air pollution testing, to help improve medical and quality of life.