The effects of orally supplemented probiotics, xylooligosaccharides and Trametes versicolor extracellular polysaccharopeptides on inflammatory response and gut microbiota in parenterally-fed rats with short bowel syndrome

• Main Authors: Chen, Chun-Chiao, 陳純巧
• Other Authors: LO, HUI-CHEN
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/umu8eu

碩士 === 輔仁大學 === 營養科學系碩士班 === 107 === Patients with short bowel syndrome (SBS) require total parenteral nutrition (TPN) to sustain their life; however, long-term use of TPN may result in intestinal atrophy, immune dysfunction, bacterial translocation and further lead to bacteremia. Studies showed that probiotics have the abilities to maintain intestinal integrity and modulate immune response. In addition, prebiotics can be fermented by probiotics to produce short chain fatty acids that provide energy for the epithelial cells of the intestine. Moreover, Trametes versicolor extracellular polysaccharopeptides (TV-ePSP) have anti-inflammatory and immune modulatory activities. In the present study, we aimed to investigate the effects of single or simultaneous, oral supplementations of probiotics, xylooligosaccharides (XOS) and TV-ePSP on inflammatory response and gut microbiota in SBS rats with TPN infusion. Male SD rats, except those in the control group, were performed with bowel resection from 10 cm beyond the ligament of Treitz to 5 cm proximal colon and catheterization in the right jugular vein. One day after the surgeries, the SBS rats were orally administered with deionized water, probiotics (10 9 CFU of Lactobacillus acidphilus and 10 9 CFU of Bifidobacterium bifidum), TV-ePSP (1 g/kg), probiotics and TV-ePSP, probiotics and XOS (500 mg/day) or the combination of probiotics, XOS and TV-ePSP daily for 6 days. The results showed that single supplementation of probiotics significantly decreased the SBS-increased plasma nitrite/nitrate, jejunal damage, hepatic interferon (IFN)-r, pulmonary interleukin (IL)-8, and the liver, kidney and spleen weights; single supplementation of TV-ePSP significantly decreased the SBS-increased plasma plasma nitrite/nitrate and immunoglobulin-A and jejunal damage and IFN-r, as well as increased the SBS-decreased villus height in the jejunum; combination of probiotics and TV-ePSP significantly increased the SBS-decreased plasma IL-6 and increased the abundance of Enterococcus genus, the lactate-producing bacteria; combination of probiotics and XOS significantly increased the SBS-decreased abundance of Clostridiales of the Firmicutes phylum; and combination of probiotics with TV-ePSP and XOS significantly increased the SBS-decreased bacterial diversity and increased the abundance of Blautia genus, butyrate-producing bacteria. In summary, combination of probiotics and TV-ePSP may have the abilities to alleviate the SBS-induced intestinal damage and inflammatory response and may increase the growth of lactate-producing bacteria.