Application study of phosphate solubilizing bacteria biofertilizer development by a biochar novel microbial immobilization method

• Main Authors: Ya-Ting Chang, 張雅婷
• Other Authors: Chien-Sen Liao
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/b54w5e

碩士 === 義守大學 === 化學工程學系暨生物技術與化學工程研究所 === 107 === The three major nutrients needed for plants are nitrogen, phosphorus and potassium. If there is a lack of nutrients in the growing environment, people will be directly applied them to the cultural mediums by the form of fertilizer. In addition to the direct application of nutrient elements, beneficial microorganisms such as nitrogen-fixing bacteria or phosphorus-dissolving bacteria can be applied as fertilizers to using their abilities of nitrogen-fixing or phosphorus-dissolving to supplement the nutrients lacking in the environments. The purpose of this study is to investigate the characteristics and effects of new microbial immobilized particles which made by the agricultural waste-rice husk mixed with Bacillus safensis and Bacillus licheniformis while them applied into the soil. B. safensis and B. licheniformis are two commercial microbial fertilizers which have the ability of phosphate-dissolving. The characteristics and effects of new microbial immobilized particles are including the soil properties, the yield and physiological changes of the text crops (Fushan lettuce, Lactuca sativa Linn), the physical and chemical changes of the strain particles themselves, and biodiversity changes of soil microbial community structure after application. The goal of this study is to understand the possibility of applying this new immobilized particle to agricultural environments in the future. The results showed that 7 g of B. safensis immobilized particles with a sodium alginate concentration of 2% and 5 g of B. licheniformis immobilized particles with a sodium alginate concentration of 0.5% were added to 400 g of soil. It is best for phosphorus solubilization, crop yield, and diversity of microbial populations. In the soil analysis, the immobilized particles of B. licheniformis were applied with 5 g and 3% sodium alginate. The ratio of available phosphorus to total phosphorus in the soil was the only condition that exceeded the control group and the direct added bacterial solution. The available phosphorus in the soil. The conversion rate was 25.8%. Compared with the initial soil, the control group (without any addition) and the added B. licheniformis solution, the available phosphorus in the soil increased by 6.81%, 20.37%, and 2.52%, respectively. The analysis of soil microbial populations showed that the diversity of Shannon, Simpson and other diversity indicators was observed to be higher than that of other groups. From the bar plot results of the bacterial phase distribution, the distribution of the bacterial phase of S7, which the soil was added with B. licheniformis and fixed with 2% sodium alginate concentration before planting, was significantly different from that of the other groups.