Analyzing of Major Active Components in Daphne genkwa Using Supercritical Carbon Dioxide Extraction

• Main Author: 許致凱
• Other Authors: WU,CIAO-SONG
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/cgqv2r

碩士 === 明志科技大學 === 化學工程系碩士班 === 107 === Abstract Daphne genkwa as a common traditional Chinese herbal medicine, its flower has the functions of water removal and clear sputum and has been used in Taiwan for a long time. It is often used to revitalize the body. Many scholars in modern times have studied in depth various aspects. Most of its extraction uses traditional solvent extraction as research, At present, no literature has reported the use of supercritical carbon dioxide to extract Daphne genkwa, and to explore its active ingredients. This study explored the extraction conditions in the following conditions: pressure at 200 bar, 250 bar, 300 bar, temperature at 313 K, 323 K, 333 K, carbon dioxide flow rate at 2 L/min, 3 L/min to influence the rate of extraction. The sample was dissolved in methanol and was analyzed by using a MetaChem C18-A(250×4.6mm,5μm) column with a gradient elution composed of 5％ acetic acid aqueous solution and methanol(40/60， v/v). Flow rate 1mL/min and chromatographic temperature 25 °C tube. Under this condition, the peaks of the apigenin was identified by comparing the retention time with that of the standard reagent. Typical retention time of Apigenin was 7min. The best operation coudition of extraction Apigenin is in pressure 250 bar, temperature 333 K, and CO2 flow rate kept at 2L/min.