Down regulation of miR-29a by Astragalus mongholicus treatment in Colorectal Cancer

• Main Authors: CHIEN-SHUO YU, 余乾碩
• Other Authors: LI-JEN SU
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/5pk9y6

碩士 === 國立中央大學 === 系統生物與生物資訊研究所 === 107 === Astragalus Mongholicus (AM), has pointed out in the past several articles that it has effect on the treatment of colorectal cancer, but the molecular mechanism of the part is still unclear. The purpose of this study is to find out the molecular mechanism of the influence of jaundice on colorectal cancer cells and tissues, and to study how Astragalus mongholicus affects or treats colorectal cancer from the perspective of molecular biology. This study begins with the analysis of the gene chip, uses the GeneSpring software GX version 7.3 (Agilent, CA, USA) to select the differential genes first, and uses the miRTarBase biological database to combine the analysis, and finally combines the previous research results of the laboratory to select the candidate genes. --- miR-29a, a non-coding RNA for subsequent experiments. In this study, the real-time polymerase chain reaction assay was used to prove that the HCT116 cell line of colorectal cancer and the 24 Rats left by the graduate students Zeng Heather in this laboratory were injected subcutaneously into HCT116 cells and grew tumors, and then received jaundice. Drug treatment, and finally in the treatment of tumor tissue. The amount of miR-29a in the tissue is affected by the inhibition caused by jaundice. Later, because this study has cooperated with Chen Wen yih laboratory, this study obtained the right to use Chen's patented nDNA modified probe for in situ hybridization experiments. We can confirm that the expression of miR-29a in HCT116 cell line is different due to the addition of jaundice. After this experiment, the mouse tissue array wafer left by the previous laboratory was used for histological in situ hybridization experiment, and further proved that after the administration of jaundice, the miR caused by the administration of jaundice can also be seen in histology. -29a inhibition of the amount of expression. Perhaps in the future, miR-29a's target drugs will be combined with Astragalus to develop new medical and commercial uses for the treatment of colorectal cancer.