Study of Earth Microbiome: Treatment of mice with High-GABA rice induced by endophytic bacteria increases neuropeptide Y and reduces anxiety

• Main Authors: Enkhbat Zayabaatar, 薩恩貝
• Other Authors: Eric Huang
• Format: Others
• Language: en_US
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/kxr5e6

碩士 === 國立中央大學 === 生醫科學與工程學系 === 107 === Abstract Background: Endophytic bacteria are defined as bacteria detected inside surface-sterilized plants or extracted from inside plants without causing apparent disease or no harmful effects on the plants. Neuropeptide Y (NPY) is a suppressive neurotransmitter that can be secreted from both the central and peripheral neurons. It has been previously demonstrated that the level of NPY can be altered in several animal models of depression, as well as be differentially regulated by acute and repeated stress. Aim: The goal of this study is to test our hypothesis that high-value rice can be generated by colonization of endophytic bacteria. Methods: Bacillus amyloliquefaciens (BA) was used as an endophytic bacterium. NPY concentrations in plasma of ICR mice were measured by the enzyme-linked immunosorbent assay (ELISA). Furthermore, a model of a hole-board test was used to evaluate the anxiety-related behavior in mice. Results: Rice colonized with BA bacteria for ten days increased the content of in gamma-aminobutyric acid (GABA). The amount (9.57 ± 0.52 ng/ml) of NPY in the plasma of mice fed with high-GABA rice was significantly higher than that (6.82 ± 0.28 ng/ml) in mice fed with control rice. Moreover, less anxiety-related behavior was observed in mice fed with high-GABA rice in comparison with mice fed with control rice. Conclusion: Bacillus amyloliquefaciens (BA) functions as an endophytic bacterium to yield a high GABA rice that can upregulate the plasma level of NPY for reduction of the anxiety in mice. Keywords: neuropeptide Y (NPY), gamma-aminobutyric acid (GABA), anxiety, Bacillus amyloliquefaciens, GABA(b) receptor antagonist, ultrasonic conditioning vocalization, hole-board test