| Summary: | 碩士 === 國立宜蘭大學 === 生物機電工程學系碩士班 === 107 === Anthropogenic noise has been recognized as a major source of environmental pollution, including frequent shipping, sonar detection, generator sets and river engineering, which have been shown to have a variety of negative impacts on organisms. This study uses sinusoidal waves to stimulate Scylla serrata, and records behavior and electrocardiogram to explore whether sinusoidal noise has a specific frequency band that can significantly affect the behavior and heart rate of Scylla serrata. The experiment was divided into two batches: the batch of experiments (O1~O6) which noise stimulations were shifted from low to high in the order of eight frequencies of 100 Hz, 400 Hz, 700 Hz, 1000 Hz, 1300 Hz, 1600 Hz, 1900 Hz, and 2200 Hz. The second batch of experiments (R1~R6) which noise stimulations were randomed from the 8 frequencies of the first batch of experiments. The experimental results showed that the behavior, under the 100 Hz, the proportion of crab moving after sound playback is greater than other higher frequencies. Although there is no statistically significant difference, in most of the stimulation frequencies, the behavior of the first stimulation is more intense. The heart rate after stimulation is decreased, which can be inferred. When the environmental sound changes, the internal physiology also changes synchronously. It was also found in the experiment that the rate of decline in heart rate was higher than that of the second and third stimulations during the first stimulation, which may be due to the adaptation of crab to sound.
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