Effect of Storage Condition on Spore Viability in Pentarhizidium orientale

• Main Authors: LEE, PEI-CHUN, 李佩純
• Other Authors: CHEN, TZE-YING
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/9r78u5

碩士 === 國立宜蘭大學 === 生物資源學院碩士在職專班 === 107 === Pentarhizidium orientale (Hook.) Hayata (= Matteuccia orientalis (Hook.) Trevis.) was a national critically endangered (NCR) fern of Taiwan, which needs to be protected by ex situ conservation using spore culture, gradually decreases - population size because of human interference. Fern spore is usually used for propagation, and establishing spore bank is an assistant method of ex situ conservation. However, P. orientale is a fern with short-lived chlorophyllous spore. Thus, an efficient and easy storage treatment to elongate the span of spore viability of P. orientale was needed. In this study, the germination rates of spores, if being capsuled by pinnule or not at four different temperature (-80℃, -20℃, 4℃, 25℃) were monitored, at 2 weeked-interval until 16 weeks. Therefore, life-span of spores was calculated based on germination rates. Results showed that life span of the spores without pinnule capsule at four different storage temperature is less than 1 week. In contrast, spores covered with pinnule capsule, the longest life span of the spores is ca. 10 weeks at 4℃, the second one is 6 weeks at -20℃, the one at 25℃ is 3 weeks, but the spores stored at -80℃ lost viabilities in a short time. In general, the best storage condition for maintaining spore viability was stored the spores covering with pinnule capsule at 4℃.