Anti-inflammatory effects and heavy metal content of Ginseng radix residue extracts

碩士 === 國立宜蘭大學 === 生物資源學院碩士在職專班 === 107 === Since ancient times, ginseng has been awarded the title of "King of the King of Medicine", and it is a precious medicinal material in the prescription of Chinese medicine. The composition and pharmacological mechanism of ginseng have been studied...

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Bibliographic Details
Main Authors: HUANG,HUAI-DE, 黃懷德
Other Authors: HSU,HUI-CHEN
Format: Others
Language:zh-TW
Published: 2019
Online Access:http://ndltd.ncl.edu.tw/handle/taxrp4
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Summary:碩士 === 國立宜蘭大學 === 生物資源學院碩士在職專班 === 107 === Since ancient times, ginseng has been awarded the title of "King of the King of Medicine", and it is a precious medicinal material in the prescription of Chinese medicine. The composition and pharmacological mechanism of ginseng have been studied and discussed. Therefore, this study is mainly aimed at domestic Chinese herbal medicine. A large amount of ginseng drug residue is produced in the industry. The application of the drug residue to the animal feed requires consideration of the feeding properties of the animal. The analysis results show that the nutrient neutral detergent fiber (23.10±0.28%) and the acid detergent fiber (12.45±0.07%) )) Suitable for further development into feeding animals or as an additive. The anti-inflammatory analysis was carried out in the extract of the drug standard. In this study, the mouse anti-inflammatory response test was performed using mouse macrophage RAW264.7 as a test cell, and LPS-induced mouse macrophage RAW264.7 produced an inflammatory response when generated. A large amount of nitrogen oxidizing substances can determine whether ginseng slag can effectively inhibit the inflammatory effect of cells. The experimental results show that the ginseng drug extract is more effective than the ginseng raw material and the inflammatory reaction of only adding LPS to produce nitric oxide. The substance has more inhibition of inflammatory reaction, and the ginseng drug residue extract really affects the anti-inflammatory component and mechanism of action, and further analysis experiments are needed to explore the cause. To evaluate whether the ginseng drug residue extract still has the index component. In the determination of the ginseng raw material and the ginseng drug residue, the index component of the ginseng drug residue may still be analyzed. The ginsenoside Rg1 content of the index component is analyzed by HPLC, and the residual rate of ginsenoside Rg1 in the ginseng drug residue is found after analysis. Up to 43%, in the future can think about how to completely extract ginseng active substances. In addition, the content of heavy metals remaining in the dregs after boiling water was measured. For the changes of heavy metal content in different boiling times, the experimental results showed that the heavy metal content was not exceeded due to the change of boiling time, and all were within the national safety standards. The heavy metals of cadmium and copper are boiled for a long time, and the heavy metals are concentrated into the dregs. The analysis of zinc metal content will increase after boiling for 1 hour, and then the longer the boiling time, the ingredients will be free from the soup. The cause of the change in the results of heavy metal experimental analysis still needs to further explore the factors that should be affected.