| Summary: | 碩士 === 國立高雄師範大學 === 生物科技系 === 107 === In addition to producing next generation, flowering keeps genetic diversity of Angiosperm, is the most important phase in their life cycle. “Florigen” is known as the effective signaling factor in the flowering pathway, to understand the relation between florigen and environmental factors, seems like a potential way to ameliorate agricultural cultivation and control flowering. Guzmania is the major important bromeliads potted plants in southern Taiwan, including Kaohsiung and Pingtung. However, the ‘Natural flowering’ of immature plants caused by exposure under low temperature could induce floral initiation and non-synchronous flowering, seriously affecting flower quality. Previous studies have found the reproductive habits of genus Guzmania are biased towards C3 plants, so in this study, the flowering regulation pathway of C3 plant was used as a template, and the Guzmania ‘Cherry’ mature plants with 25-30 leaves were selected and treated with low temperature, light or GA3. The results showed that plants through 2-3days of cold temperature (below12°C) and sampling after one month, the FT relative gene expression in the shoot was 3.8 times higher than control, the FT relative gene expression in the fourth leaf was 1.5 times higher than control. Plants treated with 10°C for 14 days in the growth chamber, the FT relative gene expressions in shoot both grew in light and dark were significantly higher than control, and the dark group was found to be higher than the light group. Treated with 20 ppm GA3 for 3days and sampling after 2weeks, the FT relative gene expression of dark group in the fourth leaf was higher than the light group. Furthermore, treated with 100 ppm GA3, and the other additional treated with GA inhibitor chlormequat chloride (ccc) can inhibit the FT relative gene expression significantly in shoot top. Plants grew in light and dark via ethylene treated, the FT relative gene expression of the shoot both grew in light were significantly higher than control, and also was significantly higher than the dark group.
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