| Summary: | 碩士 === 國立高雄師範大學 === 生物科技系 === 107 === Tillering is an important determining factor on crop plants yields. Breeding methods for crop yield improvement by manipulating tillering has not been achieved, because of the poor knowledge of these factors controlled tillering (branching) growth and development. Tillering of Gramineae crops is controlled by a major and recessive gene, and complex interactions among genes that related to plant endogenous hormone and environmental factors (light, temperature and water). Sorghum (Sorghum spp.) is an important crops in the world, and use for cereals, fodder and energy materials. Forage sorghum is whole plant utilization, tillering trait seriously affecting production, so the objectives of this study was to understand that relation and interaction among the Dormancy-associated 1 gene of sorghum (SbDRM1), More Axillary growth 2 gene of sorghum (SbMAX2) and the Teosinte Branched 1 of sorghum (SbTB1) during the seedlings of non-tillering (single high stem) type and tillering type sorghum varieties. First we confirmed these 3 gene sequences present in our material sorghum by genomic DNA PCR. The expression of SbTB1 was analyzed by RT-PCR sampling from stem base and young leaves of the 10 to 22 days old seedlings. The results showed that no SbTB1 expression in non-tillering type until 22D, but both SbDRM1and SbMAX2 gene were sustained expression during whole seedling stage. No SbTB1 expression until 17D after germination in tillering type sorghum, no SbDRM1 expression in 10D after germination, until 17D, and SbMAX2 constant expression during the seedling growth.
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