| Summary: | 碩士 === 國立中山大學 === 生物科學系研究所 === 107 === Salmonella is a Gram-negative, facultative anaerobe that is one of the common bacteria that causes salmonellosis. Salmonella has antitumor potentials and targeting tumor activity. Heparanase is an endo-β-D-glucuronidase, which can cleave heparin sulfate on the cell surface. Heparanase can destroy the extracellular matrix and involve in tumor metastasis and angiogenic activity. Previously, Salmonella had been demonstrated to inhibit tumor metastasis. It is still unclear about whether Salmonella can regulate the expression of heparanase. Herein, the expression of heparanase in tumor cells was decreased after treatment with Salmonella, and cell migration assay demonstrated the inhibition of cell migration after Salmonella infection. Salmonella influenced the protein levels of phosphate-protein kinase B (P-AKT) and phosphate-extracellular regulated protein kinases (P-ERK) which are known to be involved in the expression of heparanase. The anti-tumor metastatic effects of Salmonella were evaluated in mice bearing experimental metastasis tumor model. Compared with the control group, Salmonella significantly prolonged animal survival and reduced the metastatic nodules. Our study indicates that Salmonella plays a vital role in the inhibition of tumor metastasis through the down-regulation of heparanase.
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