Salmonella inhibits tumor metastasis by down-regulation heparanase
碩士 === 國立中山大學 === 生物科學系研究所 === 107 === Salmonella is a Gram-negative, facultative anaerobe that is one of the common bacteria that causes salmonellosis. Salmonella has antitumor potentials and targeting tumor activity. Heparanase is an endo-β-D-glucuronidase, which can cleave heparin sulfate on the...
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ndltd-TW-107NSYS51120102019-09-17T03:40:11Z http://ndltd.ncl.edu.tw/handle/t6w472 Salmonella inhibits tumor metastasis by down-regulation heparanase 沙門氏菌藉由調解乙醯肝素酶的表現抑制腫瘤轉移 Wen-Yi Chiou 邱宛怡 碩士 國立中山大學 生物科學系研究所 107 Salmonella is a Gram-negative, facultative anaerobe that is one of the common bacteria that causes salmonellosis. Salmonella has antitumor potentials and targeting tumor activity. Heparanase is an endo-β-D-glucuronidase, which can cleave heparin sulfate on the cell surface. Heparanase can destroy the extracellular matrix and involve in tumor metastasis and angiogenic activity. Previously, Salmonella had been demonstrated to inhibit tumor metastasis. It is still unclear about whether Salmonella can regulate the expression of heparanase. Herein, the expression of heparanase in tumor cells was decreased after treatment with Salmonella, and cell migration assay demonstrated the inhibition of cell migration after Salmonella infection. Salmonella influenced the protein levels of phosphate-protein kinase B (P-AKT) and phosphate-extracellular regulated protein kinases (P-ERK) which are known to be involved in the expression of heparanase. The anti-tumor metastatic effects of Salmonella were evaluated in mice bearing experimental metastasis tumor model. Compared with the control group, Salmonella significantly prolonged animal survival and reduced the metastatic nodules. Our study indicates that Salmonella plays a vital role in the inhibition of tumor metastasis through the down-regulation of heparanase. Che-Hsin Lee 李哲欣 2019 學位論文 ; thesis 55 zh-TW |
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碩士 === 國立中山大學 === 生物科學系研究所 === 107 === Salmonella is a Gram-negative, facultative anaerobe that is one of the common bacteria that causes salmonellosis. Salmonella has antitumor potentials and targeting tumor activity. Heparanase is an endo-β-D-glucuronidase, which can cleave heparin sulfate on the cell surface. Heparanase can destroy the extracellular matrix and involve in tumor metastasis and angiogenic activity. Previously, Salmonella had been demonstrated to inhibit tumor metastasis. It is still unclear about whether Salmonella can regulate the expression of heparanase. Herein, the expression of heparanase in tumor cells was decreased after treatment with Salmonella, and cell migration assay demonstrated the inhibition of cell migration after Salmonella infection. Salmonella influenced the protein levels of phosphate-protein kinase B (P-AKT) and phosphate-extracellular regulated protein kinases (P-ERK) which are known to be involved in the expression of heparanase. The anti-tumor metastatic effects of Salmonella were evaluated in mice bearing experimental metastasis tumor model. Compared with the control group, Salmonella significantly prolonged animal survival and reduced the metastatic nodules. Our study indicates that Salmonella plays a vital role in the inhibition of tumor metastasis through the down-regulation of heparanase.
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author2 |
Che-Hsin Lee |
author_facet |
Che-Hsin Lee Wen-Yi Chiou 邱宛怡 |
author |
Wen-Yi Chiou 邱宛怡 |
spellingShingle |
Wen-Yi Chiou 邱宛怡 Salmonella inhibits tumor metastasis by down-regulation heparanase |
author_sort |
Wen-Yi Chiou |
title |
Salmonella inhibits tumor metastasis by down-regulation heparanase |
title_short |
Salmonella inhibits tumor metastasis by down-regulation heparanase |
title_full |
Salmonella inhibits tumor metastasis by down-regulation heparanase |
title_fullStr |
Salmonella inhibits tumor metastasis by down-regulation heparanase |
title_full_unstemmed |
Salmonella inhibits tumor metastasis by down-regulation heparanase |
title_sort |
salmonella inhibits tumor metastasis by down-regulation heparanase |
publishDate |
2019 |
url |
http://ndltd.ncl.edu.tw/handle/t6w472 |
work_keys_str_mv |
AT wenyichiou salmonellainhibitstumormetastasisbydownregulationheparanase AT qiūwǎnyí salmonellainhibitstumormetastasisbydownregulationheparanase AT wenyichiou shāménshìjūnjíyóudiàojiěyǐxīgānsùméidebiǎoxiànyìzhìzhǒngliúzhuǎnyí AT qiūwǎnyí shāménshìjūnjíyóudiàojiěyǐxīgānsùméidebiǎoxiànyìzhìzhǒngliúzhuǎnyí |
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