| Summary: | 博士 === 國立清華大學 === 生物科技研究所 === 107 === Mitochondria are critical organelles because they produce ATP by oxidative phosphorylation and regulate survival through miscellaneous metabolisms. Mitochondria morphology changes continuously through fission, fusion and movement by various mechanisms. Disturbance of mitochondria dynamics is associated with neuronal and cardiac diseases. Mitochondria dynamics have been found and studied for decades; however, the complicated mechanisms are not fully dissolved. The goal of the study is to elucidate the underlying regulatory mechanism of mitochondria dynamics. We identified Srv2 that interacts with mitochondria fission protein Dnm1 by genetic screen. Srv2 is a highly conserved protein that regulates actin dynamics and cAMP/ PKA signaling. Our data demonstrated that Srv2 interacts with Dnm1 specifically on mitochondria. Genetic manipulation results indicated Srv2 contributes to mitochondria dynamics through its function on actin dynamics. In addition, Srv2 depletion negatively affects mitochondria respiratory activity. Our results suggest that Srv2 mediates mitochondria dynamics through its role in conjugating the fission machinery and actin assembly. This work characterized a novel pro-fission factor, and unveiled the link between mitochondria and cytoskeleton system.
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