Multifunctional Nanoparticles and Applications in Biosensing
碩士 === 國立臺灣大學 === 化學研究所 === 107 === The first part of thesis is focused on the preparation of gold nanosphere particle. Nanosphere particle SERS tags with core-molecular-shell (CMS) structure that embedded Raman reporter in SERS tags to circumvent disturbance of non-targeted molecules has been found...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2019
|
| Online Access: | http://ndltd.ncl.edu.tw/handle/33hgq6 |
| Summary: | 碩士 === 國立臺灣大學 === 化學研究所 === 107 === The first part of thesis is focused on the preparation of gold nanosphere particle. Nanosphere particle SERS tags with core-molecular-shell (CMS) structure that embedded Raman reporter in SERS tags to circumvent disturbance of non-targeted molecules has been found to be an effective tool in biological sensing. Herein, we report our results of using CMS SERS tags for the detection of Enterovirus 71 (EV71). We first optimized the CMS SERS tags with various conditions including materials of CMS, thickness of CMS and reaction time. Our results show that 10 nm thickness of gold shell of SERS tags offers the highest SERS signals. EV71 antibody conjugated CMS SERS tags is used for detection of EV71 antigen. The correlation between SERS intensity of SERS tags and concentration of EV71 antigen is established. The detection limit is found to be 1.33 g/mL. In the second part of the thesis, we attempted to develop a SERS-active nano-sized magnetic stir bars which can potentially accelerate the process of antigen detection. We demonstrated that various sizes of stir bars can be prepared. The fast response of nano-sized stir bars to magnetic stirrer was validated under microscope. Results demonstrate that strong SERS activity can be generated by this magnetic response platform. This study provides a novel strategy that can be possibly used for rapid detection of SERS.
|
|---|