Projector-based 3D printing for pcMSC cell sheets

• Main Authors: Hung-Hao Chang, 張紘豪
• Other Authors: Po-Ling Kuo
• Format: Others
• Language: en_US
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/nardgz

碩士 === 國立臺灣大學 === 生醫電子與資訊學研究所 === 107 === In this article described a method which basing on 3D printing to producing stem cell sheets by using projector. This method included the 3D printing machine, the manufacture for printing gel and the stem cell culture. The printing gel is exposed and solidified by light projector system. The light projector system was remodeled from the commercial projector. Moreover, the outlook of the gel can be designed by the exposed pattern from projector. The printing gel is comprised by Gelatin Methacryloyl(GelMa), Lithium Phenyl(2,4,6-trimethylbenzoyl)phosphinate (LAP). In the cell culture, I choose the human placenta chorionic decidual-derived MSCs(pcMSCs). It can adhere and culture on the gel. In future work, because of the features of 3D printing, the gel can be designed with porous structure. This structure can allow the medium flowing faster in the gel. The final product, pcMSC cell sheets, completed by this method, can be used in clinical research and severe burn wound treatment.