| Summary: | 碩士 === 國立臺灣大學 === 材料科學與工程學研究所 === 107 === The goal of this research is to design a peptide-based polyelectrolyte containing neuronal stimulant (i.e., glutamic acid) for the fabrication of electroactive 3D fibrous scaffold with aligned fibers for neural tissue engineering.
The polypeptides are designed based on biocompatible poly(γ-benzyl-L-glutamate) (PBG), which can be synthesized easily by conventional ring opening polymerization reaction. Partial hydrolysis of the benzyl groups in PBG yields a random copolymer of poly(γ-benzyl-L-glutamate)-r-poly(α-L-glutamic acid) (PBGA). By using electrospinning technique, we fabricate PBGA fibrous scaffold with aligned fibers. The scaffold made of peptide-based polyelectrolyte, sodium salt of poly(γ-benzyl-L-glutamate)-r-poly(α-L-glutamic acid) (PBGA-Na), is obtained by reacting the COOH groups in PBGA scaffold with NaOH aqueous solution.
The PBGA-Na with 20 mol% of COO-Na+ side chains (i.e., PBGA20-Na) is water-insoluble, which can maintain the aligned structure of the fibrous scaffold and stimulate neurite outgrowth in alignment. Hence, in this thesis, we focus on culturing neurons on this aligned, electroactive and neuronal stimulant-contained fibrous scaffold. We also discuss the proliferation and differentiation of neurons on this scaffold with or without electrical stimulation.
The biocompatibility of PBG, PBGA20 and PBGA20-Na are significantly better than polycaprolactone (PCL). Furthermore, the integration of neuronal stimulant into polypeptides (i.e., PBGA20 and PBGA20-Na) shows an enhancement of cell adhesion, proliferation and differentiation. Cells on all the materials tested in this thesis extend longer neurites with electrical stimulation than without stimulation. According to the results of the experiments, all the scaffolds with the aligned fibers can promote the growth of neurites along. Neurite outgrowth on the electroactive polypeptide containing glutamic acid (i.e., PBGA20-Na) is the longest among the four materials. In conclusion, PBGA20-Na is a unique and promising biomaterial for neural tissue engineering.
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