| Summary: | 碩士 === 國立臺灣大學 === 醫學工程學研究所 === 107 === Cells in their native environment have various morphologies, which are reflected in cytoskeletal structures. In addition, due to physiological tissue movements, mechanical strains applied to cells are often anisotropic. Studies have shown that anisotropic loading change cytoskeleton architecture, focal adhesion organization, and gene expression. YAP is a mechano-response transcriptional coactivator that translocates to the nucleus when activated. We hypothesize that anisotropic mechanical loading results in a direction-dependent YAP response, which may contribute to morphology-dependent gene expression changes. Microcontact printed cells in capsular and linear patterns were subjected to the uniaxial cyclic stretch. We found YAP nuclear translocation only occurred in parallelly stretched linear cells. Furthermore, our results indicated that cell shape, myosin Ⅱ, and zyxin regulate the anisotropic YAP mechanosensing. Our study provides new insights to study the relationship between zyxin and myosin in anisotropic YAP mechanotransduction.
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