| Summary: | 碩士 === 中國文化大學 === 生物科技研究所 === 107 === Sudan is a carcinogenic industrial azo-dye and is restricted for use in food. However, it has been detected in food on several occasions, such as in paprika and in egg yolk. This study was designed to simulate the transfer of Sudan to eggs and tissues after its administration to laying hens. Three groups of laying hens received feed containing Sudan IV at 0 mg/kg, 4mg/kg and 40 mg/kg. One week after feeding, normal feeding was resumed, and eggs were collected for egg quality analysis, blood, liver, fat and eggs were collected and analyzed for the concentrations of Sudan and it’s metabolites. The results showed that there was no significant difference in egg weight, eggshell strength, and Haugh unit. In the high-dose group, Roche color fan scale reached 15 on the second day of feeding and returned to normal at 20 days after withdrawn from feed. The highest level of Roche color fan scale in the low-dose group reached 12 and returned to normal at 14 days after withdrawn from feed. The highest Sudan concentration in the egg yolk was 2.78 and 0.7 mg/kg in the high-dose and low-dose treatment groups, respectively. At 20 days after withdrawn from feed, Sudan IV and its metabolites were not detected in egg yolk, liver, blood and fat. In conclusion, findings of ow study suggests that the resume of egg color seems to correlate with the clearance rate of sudan in tissues. Thus, observation of egg yolk color change might be a quicker and simple way to determine the clearance of Sudan after its contamination in eggs. However, residue concentration still need to be analyzed by certain high tech equipment to ensure the safeness of the food.
|
|---|
