| Summary: | 碩士 === 中國文化大學 === 生物科技研究所 === 107 === Fipronil is a broad-spectrum insecticide that was widely used in environmental sanitation or animal medicine for the prevention and treatment of pet fleas. It is also found misused in laying farm occasionally and would lead to food safety problem. In this study, twenty-seven laying hens (nine per treatment) were given fipronil at zero, 1 mg/kg bw and 3 mg/kg bw via oral gavage. Eggs were collected every day for egg quality analysis. Blood, liver, fat and muscle tissues were collected weekly, then analyzed for concentrations of fipronil and its metabolites by UPLC-MS/MS. All tissue samples (eggs, blood, liver, fat, muscle) were pre-treated with acetonitrile, then performed metabolite analysis by UPLC-MS/MS. Results showed that egg weight, eggshell strength and Haugh unit were not different among treatments. Fipronil and its metabolites were undetectable in all tissues at 14 days after treatments. In conclusion, fipronil, if accidentally consumed by laying hens, can be metabolized and absent from tissues, organs and eggs in two weeks.
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