Effects of Cordyceps militaris fruiting body on anti-fatigue in ICR mice

• Main Authors: FEI, HAO-YUAN, 費皓元
• Other Authors: LIANG, CHIN-HUNG
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/uppnsh

碩士 === 東海大學 === 食品科學系 === 107 === Fatigue is caused by labor or excessive work, and is closely related to many occupational disasters; therefore, how to reduce the fatigue of workers and occupational injuries has become an important issue. Fatigue refers to the inability of the body to show its optimal state, caused by subjective feelings, tissue damage or energy shortages; the length of the recovery period varies depending on the level of exercise and supplementation of nutrients.Cordyceps militaris is a genus of Cordyceps, containing cordycepin, adenosine, mannitol and polysaccharides. Studies have indicated that Cordyceps militaris has many physiological functions such as anti-diabetes, blood fat reduction and liver protection. At present, among the anti-fatigue functional health products approved by the Food and Drug Administration of the Ministry of Health and Welfare, there are three types of mushroom products, two of which are Cordyceps sinensis mycelium products of Cordyceps. Studies have shown that Cordyceps militaris has more functional components than Cordyceps sinensis. At present, little research has been conducted on the anti-fatigue efficacy of Cordyceps militaris fruiting bodies. Therefore, the main purpose of this study is to investigate the effect of the fruiting body of Cordyceps militaris on exercise-induced fatigue. Eight weeks old male ICR mice were divided into five groups: control group, low, medium and high dose groups of Cordyceps militaris, and positive control group. The positive control group was the Cordyceps sinensis mycelium capsule certified by health food. Eight out of each group, after four weeks of feeding, exercise performance and anti-fatigue biochemical indicators analysis, sports performance is divided into forelimb grip and swimming exhaustion test.The results showed that in the forelimb grip, the experimental group (126.62 ~ 141.86 KgF) and the positive control group (146.21 KgF) fed the Cordyceps militaris fruiting body significantly improved compared with the control group (93.85 KgF); The exhaustion time of the experimental group (1267.3~1719.4 seconds) and the positive control group (1405.0 seconds) fed the Cordyceps militaris fruiting body was significantly longer than that of the control group (388.1 seconds). In addition to anti-fatigue blood biochemical indicators, the results show that in the blood glucose, the high dose group of Cordyceps militaris can increase the blood glucose content by 1.40 times; in the case of lactate dehydrogenase, feeding the Cordyceps militaris fruiting body can reduce the dehydrogenation of lactate after exercise. The enzyme production, low, medium and high doses were reduced to 1736.3, 1541.3 and 1383.8 U / L, respectively, significantly lower than the control group (2516.3 U / L). In terms of blood ammonia, feeding the Cordyceps militaris fruiting body can reduce the blood ammonia concentration (565.9~695.4 umol/L), which is significantly lower than the control group (866.0 umol/L). In terms of creatine kinase, feeding the Cordyceps militaris fruiting body reduced creatine kinase activity, and the low, medium and high doses were reduced to 357.5, 300.0 and 278.8 U/L, respectively, which was significantly lower than the control group (903.8 U/L).In addition, no pathological changes were found in the liver, kidney and other organs of mice. There was no significant difference in the obesity index between the groups, but the total cholesterol content and low-density lipoprotein decreased when fed the Cordyceps militaris fruiting body. In summary, the Cordyceps militaris fruiting body can effectively increase the forelimb grip strength of the mice and increase the muscular endurance. It can also reduce the consumption of glucose in the body after exercise, reduce the production of fatigue-generating factors, and thus achieve the anti-fatigue effect.