Nanocomposites for Electrochemical Sensors in Human Application

• Main Authors: LI, YI-LING, 李依凌
• Other Authors: CHEN, SHENG-MING
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/8dvh4u

碩士 === 國立臺北科技大學 === 化學工程與生物科技系化學工程碩士班 === 107 === PartІ: 4-Nitroquinoline N-oxide (4-NQO) is an important biomarker based tumorigenic organic compound with high adverse effect in human body (cancer biomarker). In this study, a novel Bismuth Tungstate nanospheres Bi2WO6 decorated reduced graphene oxide (Bi2WO6/rGOS) nanocomposite have been designed through a simple, one-step sonochemical method. The as-synthesized Bi2WO6/rGOS was characterized through the HRTEM, FESEM, XPS, and XRD. Furthermore, the nanocomposite modified GCE was developed for the determination of 4-NQO for the first time. The results showed that the Bi2WO6/rGOS nanocomposite modified electrode exhibit well-defined electro-reduction peak compared to bare GCE, demonstrating the superior catalytic activity. The fabricated sensor facilitates the analysis of 4-NQO in the concentration ranges of 0.025-718 µM with a low detection limit of 6.11 nM. Further, the as-prepared Bi2WO6/rGOS/GCE has been applied to determination of 4-NQO in human blood and urine samples with satisfactory recovery results (96.39 to 98.45%), thereby showing a notable potential for extensive applications in electrochemical sensing and bio-sensing. PartП: A functional ternary nanocomposite (MnO2@NiFe2O4) with hierarchically grown flower-like core-shell assembly has been prepared through a simple two-step hydrothermal methodology. The crystallographic information of the sample was obtained via X-ray diffraction. The morphological details of spherical NiFe2O4 (core) and flower-like MnO2 (shell) were examined over FE-SEM and TEM; finally, the XPS confirms the successful formation of MnO2@NiFe2O4 ternary nanocomposite. The surface area of finely grown hierarchical MnO2 flowers on NiFe2O4 nanoparticles was measured through BET isothermal studies. The ternary nanocomposite was employed for the specific and sensitive detection of anticancer drug chlorambucil. Under the well-optimized condition, the graphically plotted calibration curve was attained at MnO2@NiFe2O4/GCE towards the detection of anticancer drug chlorambucil, which possesses the wider linear range of 0.025-574.5 µM, with the minimal detection limit of 4.68 nM. The feasibility of the sensitive layer has also been inspected on the pharmaceutical sample (oral tablets) and the biological matrix (human urine), whereas the appreciable spike recoveries were obtained. The results gotten at MnO2@NiFe2O4 NPs/GCE were also compared with the standardized HPLC method to validate the robustness of the detector. PartШ: The ZnC nanoflakes was synthesized by simple Co-precipitation method and its physicochemical properties were systematically studied with various analytical and spectroscopic techniques. Furthermore, the proposed ZnC Nfs modified with Screen-printed carbon electrode (SPCE) after that directly used for the electrochemical determination (4-NT). The ZnC/SPCE shows an excellent electrochemical reduction peak response for the (4-NT). The as prepared ZnC Nfs provide a highly selective and sensitive electrocatalyst for the (4-NT) determination with good linear range response 0.05 - 385.5µM and low detection limits 0.044µM. The ZnC/SPCE electrochemical sensor established the outstanding selectivity, well stability and reproducibility.