Fabrication of porous gelatin/chitosan hydrogel composite using 3D printing technology

• Main Authors: Huan-Hua Chang, 張桓華
• Other Authors: Kuo-Yu Chen
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/an3t6s

碩士 === 國立雲林科技大學 === 化學工程與材料工程系 === 107 === In this study, gelatin and chitosan were reacted with methacrylic anhydride to obtain photo-crosslinkable gelatin methacrylamide and chitosan methacrylamide, respectively. 1H-NMR analysis confirmed the formation of methacrylamide groups. X-ray diffraction measurements revealed that the crystallinity of gelatin and chitosan decreased after grafting of methacrylamide groups. Gelatin methacrylamide and chitosan methacrylamide were then mixed with N-vinyl-2-pyrrolidone monomer and various concentrations of photosensitizers and other additives, such as VA-086 (photo-initiator), Orange G (photo-absorber) and TEMPO (radical scavenger), to produce water-based photo-curable resin for 3D printing. The porous gelatin-chitosan hydrogels with interpenetrating polymer network structure were then fabricated by digital light processing-based 3D printer equipped with a light source of wavelength of 405 nm. The hydrogels were then post-cured under UV light and freeze-dried to obtain porous scaffolds. The experimental results demonstrated that the photo-curing rate of the resin solutions increased with the increase of the concentration of VA-086. The printing quality of the porous scaffolds was improved with the incorporation of TEMPO and Orange G. However, the photo-curing time would be prolonged. When the concentrations of VA-086, Orange G and TEMPO were 3 wt%, 70 ppm and 40 ppm, respectively, the shape and pore architecture of the 3D-printed gelatin/chitosan scaffold with a weight ratio of 9/1 were close to those of the design. The gelatin/chitosan scaffold had a lower porosity, swelling ratio and weight loss and better cell adhesion than 3D-printed gelatin scaffold. These results indicate that the 3D-printed gelatin/chitosan scaffold has a potential in customized scaffold fabrication for tissue engineering applications.