The Separation and Purification of Recombinant Proteins from Transgenic Rice Cell Culture by Electrodialysis with Filtration Membranes

• Main Authors: Chien-Yu Chang, 張倩瑜
• Other Authors: Yi-Ming Sun
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/e2s9k6

碩士 === 元智大學 === 化學工程與材料科學學系 === 107 === It is expected that electrodialysis with filtration membranes (EDFM) can effectively separate protein macromolecules based on the combined effect of molecular size and charge differences with filtration membranes and electrodialysis.A system of EDFM with proper selection of ultrafiltration (UF) membranes was established to test the feasibility of separation and purification of human serum albumin (HSA) from the medium of rice cell culture. The effects of the operating conditions such as operating voltage, current density, pH value on the performance were evaluated. The concentrations of proteins in the feed and permeate chambers were monitored by RP-HPLC. In a preliminary study, bovine serum albumin (BSA) as model, and is negatively charged with pH higher than pI in the feed solution. Therefore, BSA could be driven to the permeate side (anode) by an electric field at higher pH.BSA has higher electrophoretic coefficient (31.3 10-5 mg/V cm2 h) because of negative charge. HSA could be recovered from the medium of rice cell culture by our technology at right conditions.The results demonstrate that recovery of HSA from rice cell culture would be possible with proper selections of filtration membranes, pH, and operating electrical field by EDFM process.