Enantioselektiv HPLC-analys med kirala stationärfaser bestående av makrocykliska glykopeptider och polysackarider
The purpose of this study was to evaluate enantioselective analytical methods by separation of the enantiomers of four drugs (citalopram, zopiclone, tramadol and methylphenidate) and their metabolites. The analyses were performed with HPLC-UV with columns whose stationary phases were based on macroc...
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Linköpings universitet, Institutionen för fysik, kemi och biologi
2010
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ndltd-UPSALLA1-oai-DiVA.org-liu-573642013-01-08T13:29:41ZEnantioselektiv HPLC-analys med kirala stationärfaser bestående av makrocykliska glykopeptider och polysackaridersweEnantioselective HPLC-analysis using macrocyclic glycopeptides and polysaccharide based chiral stationary phasesBergman, CarolineLinköpings universitet, Institutionen för fysik, kemi och biologi2010EnantioselektivHPLC-analyskirala stationära fasermakrocykliska glykopeptiderpolysackariderNATURAL SCIENCESNATURVETENSKAPThe purpose of this study was to evaluate enantioselective analytical methods by separation of the enantiomers of four drugs (citalopram, zopiclone, tramadol and methylphenidate) and their metabolites. The analyses were performed with HPLC-UV with columns whose stationary phases were based on macrocyclic glycopeptides (Chirobiotic V, V2 and T) and polysaccharides (Lux Cellulose-1, Cellulose-2 and Amylose-2). The Chirobiotic V column showed high selectivity for citalopram and its metabolites. High resolution was obtained using a mobile phase consisting of methanol, acetic acid and ammonia. High selectivity for the enantiomers of zopiclone and its metabolites were obtained on the Cellulose-2 column using a mobile phase consisting of acetonitrile and ammonium acetate buffer. The enantiomers of tramadol were separated with the Amylose-2 column. However, changes in the pressure arose, probably caused by the additive NH4HCO3. When the analysis was repeated at a later occasion, reproducible results were not obtained. With the Cellulose-1 column, lower selectivity was obtained, resulting in unacceptably long analysis time. Only a few analyses of methylphenidate were performed and the results indicated that the glycopeptide columns had higher selectivity for this compound than the polysaccharides. Student thesisinfo:eu-repo/semantics/bachelorThesistexthttp://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-57364application/pdfinfo:eu-repo/semantics/openAccess |
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Enantioselektiv HPLC-analys kirala stationära faser makrocykliska glykopeptider polysackarider NATURAL SCIENCES NATURVETENSKAP |
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Enantioselektiv HPLC-analys kirala stationära faser makrocykliska glykopeptider polysackarider NATURAL SCIENCES NATURVETENSKAP Bergman, Caroline Enantioselektiv HPLC-analys med kirala stationärfaser bestående av makrocykliska glykopeptider och polysackarider |
description |
The purpose of this study was to evaluate enantioselective analytical methods by separation of the enantiomers of four drugs (citalopram, zopiclone, tramadol and methylphenidate) and their metabolites. The analyses were performed with HPLC-UV with columns whose stationary phases were based on macrocyclic glycopeptides (Chirobiotic V, V2 and T) and polysaccharides (Lux Cellulose-1, Cellulose-2 and Amylose-2). The Chirobiotic V column showed high selectivity for citalopram and its metabolites. High resolution was obtained using a mobile phase consisting of methanol, acetic acid and ammonia. High selectivity for the enantiomers of zopiclone and its metabolites were obtained on the Cellulose-2 column using a mobile phase consisting of acetonitrile and ammonium acetate buffer. The enantiomers of tramadol were separated with the Amylose-2 column. However, changes in the pressure arose, probably caused by the additive NH4HCO3. When the analysis was repeated at a later occasion, reproducible results were not obtained. With the Cellulose-1 column, lower selectivity was obtained, resulting in unacceptably long analysis time. Only a few analyses of methylphenidate were performed and the results indicated that the glycopeptide columns had higher selectivity for this compound than the polysaccharides. |
author |
Bergman, Caroline |
author_facet |
Bergman, Caroline |
author_sort |
Bergman, Caroline |
title |
Enantioselektiv HPLC-analys med kirala stationärfaser bestående av makrocykliska glykopeptider och polysackarider |
title_short |
Enantioselektiv HPLC-analys med kirala stationärfaser bestående av makrocykliska glykopeptider och polysackarider |
title_full |
Enantioselektiv HPLC-analys med kirala stationärfaser bestående av makrocykliska glykopeptider och polysackarider |
title_fullStr |
Enantioselektiv HPLC-analys med kirala stationärfaser bestående av makrocykliska glykopeptider och polysackarider |
title_full_unstemmed |
Enantioselektiv HPLC-analys med kirala stationärfaser bestående av makrocykliska glykopeptider och polysackarider |
title_sort |
enantioselektiv hplc-analys med kirala stationärfaser bestående av makrocykliska glykopeptider och polysackarider |
publisher |
Linköpings universitet, Institutionen för fysik, kemi och biologi |
publishDate |
2010 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-57364 |
work_keys_str_mv |
AT bergmancaroline enantioselektivhplcanalysmedkiralastationarfaserbestaendeavmakrocykliskaglykopeptiderochpolysackarider AT bergmancaroline enantioselectivehplcanalysisusingmacrocyclicglycopeptidesandpolysaccharidebasedchiralstationaryphases |
_version_ |
1716521682883051520 |