Characterization of FtsH proteases in the annual plant Arabidopsis thaliana
Background FtsH is an ATP-dependent membrane-bound metalloprotease. A. thaliana contains 12 FtsH proteases localized in membranes of chloroplasts and mitochondria where they form homo- or hetero-hexameric complexes. FtsH11 – the main subject of this thesis – is located in the chloroplast envelope. ...
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Umeå universitet, Kemiska institutionen
2012
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ndltd-UPSALLA1-oai-DiVA.org-umu-551612013-01-08T13:09:05ZCharacterization of FtsH proteases in the annual plant Arabidopsis thalianaengAigner, HaraldUmeå universitet, Kemiska institutionenUmeå : Umeå universitet2012Background FtsH is an ATP-dependent membrane-bound metalloprotease. A. thaliana contains 12 FtsH proteases localized in membranes of chloroplasts and mitochondria where they form homo- or hetero-hexameric complexes. FtsH11 – the main subject of this thesis – is located in the chloroplast envelope. Methods Field studies with A. thaliana to determine Darwinian fitness. A growth under outdoor conditions often allows discovering of phenotypes that are unascertainable in the controlled environment of growth chambers. Proteomic methods to discover fragments of substrate proteins (limited proteolysis) and changes in the proteome of FtsH protease deficient mutants. Results ftsh11 has increased amount of: RuBisCO activase, several Calvin cycle enzymes, two enzymes involved in starch synthesis and some chaperons. Some of those enzymes have been identified as possible substrates of FtsH11. Under long photoperiods ftsh11 develops a chlorotic phenotype accompanied by decreasing NADP+/NADPH ratio and increase of ROS damaged proteins. Doctoral thesis, comprehensive summaryinfo:eu-repo/semantics/doctoralThesistexthttp://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-55161urn:isbn:978-91-7459-445-4application/pdfinfo:eu-repo/semantics/openAccess |
collection |
NDLTD |
language |
English |
format |
Doctoral Thesis |
sources |
NDLTD |
description |
Background FtsH is an ATP-dependent membrane-bound metalloprotease. A. thaliana contains 12 FtsH proteases localized in membranes of chloroplasts and mitochondria where they form homo- or hetero-hexameric complexes. FtsH11 – the main subject of this thesis – is located in the chloroplast envelope. Methods Field studies with A. thaliana to determine Darwinian fitness. A growth under outdoor conditions often allows discovering of phenotypes that are unascertainable in the controlled environment of growth chambers. Proteomic methods to discover fragments of substrate proteins (limited proteolysis) and changes in the proteome of FtsH protease deficient mutants. Results ftsh11 has increased amount of: RuBisCO activase, several Calvin cycle enzymes, two enzymes involved in starch synthesis and some chaperons. Some of those enzymes have been identified as possible substrates of FtsH11. Under long photoperiods ftsh11 develops a chlorotic phenotype accompanied by decreasing NADP+/NADPH ratio and increase of ROS damaged proteins. |
author |
Aigner, Harald |
spellingShingle |
Aigner, Harald Characterization of FtsH proteases in the annual plant Arabidopsis thaliana |
author_facet |
Aigner, Harald |
author_sort |
Aigner, Harald |
title |
Characterization of FtsH proteases in the annual plant Arabidopsis thaliana |
title_short |
Characterization of FtsH proteases in the annual plant Arabidopsis thaliana |
title_full |
Characterization of FtsH proteases in the annual plant Arabidopsis thaliana |
title_fullStr |
Characterization of FtsH proteases in the annual plant Arabidopsis thaliana |
title_full_unstemmed |
Characterization of FtsH proteases in the annual plant Arabidopsis thaliana |
title_sort |
characterization of ftsh proteases in the annual plant arabidopsis thaliana |
publisher |
Umeå universitet, Kemiska institutionen |
publishDate |
2012 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-55161 http://nbn-resolving.de/urn:isbn:978-91-7459-445-4 |
work_keys_str_mv |
AT aignerharald characterizationofftshproteasesintheannualplantarabidopsisthaliana |
_version_ |
1716510482538430464 |