Identification of mould and blue stain fungi on wood using Polymerase Chain Reaction and Terminal Restriction Fragment Length Polymorphism
Wood inhabiting fungi oposes a great problem for preservation of wooden surfaces everywhere, being the main problem of economic losses of wooden products. A reference collection consisting of 9 different genus constituting of 21 different strains of wood-inhabiting fungi was used for identification...
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Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi
2006
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ndltd-UPSALLA1-oai-DiVA.org-uu-71002013-04-19T20:48:56ZIdentification of mould and blue stain fungi on wood using Polymerase Chain Reaction and Terminal Restriction Fragment Length PolymorphismengBijelovic, JelenaUppsala universitet, Institutionen för medicinsk biokemi och mikrobiologiUppsala : Universitetsbiblioteket2006WoodSoft rotFungiPCRT-RFLPWood fibre and forest productsTräfiber- och virkesläraWood inhabiting fungi oposes a great problem for preservation of wooden surfaces everywhere, being the main problem of economic losses of wooden products. A reference collection consisting of 9 different genus constituting of 21 different strains of wood-inhabiting fungi was used for identification of unknown species of mould and blue stain fungi on wood. The fungus DNA from the samples was isolated from malt extract agar. PCR (Polymerase Chain Reaction) was conducted on rDNA ITS1 and ITS2 regions for amplification of the DNA. The 21 samples were collected to a reference collection for identification of unknown species of fungi on wooden field samples using PCR and T-RFLP (Terminal Restriction Fragment Length Polymorphism). PCR-based methods, sequencing and T-RFLP were proven to be simple and accurate methods for detection and identification of fungi in their early stage. Student thesisinfo:eu-repo/semantics/bachelorThesistexthttp://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7100application/pdfinfo:eu-repo/semantics/openAccess |
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English |
format |
Others
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Wood Soft rot Fungi PCR T-RFLP Wood fibre and forest products Träfiber- och virkeslära |
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Wood Soft rot Fungi PCR T-RFLP Wood fibre and forest products Träfiber- och virkeslära Bijelovic, Jelena Identification of mould and blue stain fungi on wood using Polymerase Chain Reaction and Terminal Restriction Fragment Length Polymorphism |
description |
Wood inhabiting fungi oposes a great problem for preservation of wooden surfaces everywhere, being the main problem of economic losses of wooden products. A reference collection consisting of 9 different genus constituting of 21 different strains of wood-inhabiting fungi was used for identification of unknown species of mould and blue stain fungi on wood. The fungus DNA from the samples was isolated from malt extract agar. PCR (Polymerase Chain Reaction) was conducted on rDNA ITS1 and ITS2 regions for amplification of the DNA. The 21 samples were collected to a reference collection for identification of unknown species of fungi on wooden field samples using PCR and T-RFLP (Terminal Restriction Fragment Length Polymorphism). PCR-based methods, sequencing and T-RFLP were proven to be simple and accurate methods for detection and identification of fungi in their early stage. |
author |
Bijelovic, Jelena |
author_facet |
Bijelovic, Jelena |
author_sort |
Bijelovic, Jelena |
title |
Identification of mould and blue stain fungi on wood using Polymerase Chain Reaction and Terminal Restriction Fragment Length Polymorphism |
title_short |
Identification of mould and blue stain fungi on wood using Polymerase Chain Reaction and Terminal Restriction Fragment Length Polymorphism |
title_full |
Identification of mould and blue stain fungi on wood using Polymerase Chain Reaction and Terminal Restriction Fragment Length Polymorphism |
title_fullStr |
Identification of mould and blue stain fungi on wood using Polymerase Chain Reaction and Terminal Restriction Fragment Length Polymorphism |
title_full_unstemmed |
Identification of mould and blue stain fungi on wood using Polymerase Chain Reaction and Terminal Restriction Fragment Length Polymorphism |
title_sort |
identification of mould and blue stain fungi on wood using polymerase chain reaction and terminal restriction fragment length polymorphism |
publisher |
Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi |
publishDate |
2006 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7100 |
work_keys_str_mv |
AT bijelovicjelena identificationofmouldandbluestainfungionwoodusingpolymerasechainreactionandterminalrestrictionfragmentlengthpolymorphism |
_version_ |
1716582817260896256 |