Correlation between Fertilization, Cleavage and Pregnancy Rate with Sperm DNA-Fragmentation Index (DFI)
The chromatin integrity in sperm cells is vital for successful pregnancy. In this study DNA-damage was evaluated in sperm cells from 50 men attending In Vitro Fertilization (IVF) or Intra Cytoplasmic Sperm Injection (ICSI) treatment. Male semen samples were purified with a two-shift gradient before...
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Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi
2008
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ndltd-UPSALLA1-oai-DiVA.org-uu-92042013-01-08T13:17:07ZCorrelation between Fertilization, Cleavage and Pregnancy Rate with Sperm DNA-Fragmentation Index (DFI)engNymo, KaitlinUppsala universitet, Institutionen för medicinsk biokemi och mikrobiologiUppsala : Universitetsbiblioteket2008Human spermmale infertilitySperm Chromatin Dispersion TestDNA-damagein vitro fertilization.BiochemistryBiokemiThe chromatin integrity in sperm cells is vital for successful pregnancy. In this study DNA-damage was evaluated in sperm cells from 50 men attending In Vitro Fertilization (IVF) or Intra Cytoplasmic Sperm Injection (ICSI) treatment. Male semen samples were purified with a two-shift gradient before the sperm cells were treated with the Halosperm® Test Kit and evaluated for DNA-damage. The samples were divided in two groups according to DNAFragmentation Index (DFI) of 30 % and the results correlated with fertilization, cleavage and pregnancy rate. Men with DFI ≥ 30 % had a higher fertilization and pregnancy rate and a lower cleavage rate compared to men with DFI ≤ 30 %. The conclusions were that fertilization in vitro may be independent of the degree of DNA-damage, the embryonic development could be seriously disrupted by damaged sperm cells, and the pregnancy rate showed no correlation to a DFI threshold of 30 %. Student thesisinfo:eu-repo/semantics/bachelorThesistexthttp://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9204application/pdfinfo:eu-repo/semantics/openAccess |
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English |
format |
Others
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Human sperm male infertility Sperm Chromatin Dispersion Test DNA-damage in vitro fertilization. Biochemistry Biokemi |
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Human sperm male infertility Sperm Chromatin Dispersion Test DNA-damage in vitro fertilization. Biochemistry Biokemi Nymo, Kaitlin Correlation between Fertilization, Cleavage and Pregnancy Rate with Sperm DNA-Fragmentation Index (DFI) |
description |
The chromatin integrity in sperm cells is vital for successful pregnancy. In this study DNA-damage was evaluated in sperm cells from 50 men attending In Vitro Fertilization (IVF) or Intra Cytoplasmic Sperm Injection (ICSI) treatment. Male semen samples were purified with a two-shift gradient before the sperm cells were treated with the Halosperm® Test Kit and evaluated for DNA-damage. The samples were divided in two groups according to DNAFragmentation Index (DFI) of 30 % and the results correlated with fertilization, cleavage and pregnancy rate. Men with DFI ≥ 30 % had a higher fertilization and pregnancy rate and a lower cleavage rate compared to men with DFI ≤ 30 %. The conclusions were that fertilization in vitro may be independent of the degree of DNA-damage, the embryonic development could be seriously disrupted by damaged sperm cells, and the pregnancy rate showed no correlation to a DFI threshold of 30 %. |
author |
Nymo, Kaitlin |
author_facet |
Nymo, Kaitlin |
author_sort |
Nymo, Kaitlin |
title |
Correlation between Fertilization, Cleavage and Pregnancy Rate with Sperm DNA-Fragmentation Index (DFI) |
title_short |
Correlation between Fertilization, Cleavage and Pregnancy Rate with Sperm DNA-Fragmentation Index (DFI) |
title_full |
Correlation between Fertilization, Cleavage and Pregnancy Rate with Sperm DNA-Fragmentation Index (DFI) |
title_fullStr |
Correlation between Fertilization, Cleavage and Pregnancy Rate with Sperm DNA-Fragmentation Index (DFI) |
title_full_unstemmed |
Correlation between Fertilization, Cleavage and Pregnancy Rate with Sperm DNA-Fragmentation Index (DFI) |
title_sort |
correlation between fertilization, cleavage and pregnancy rate with sperm dna-fragmentation index (dfi) |
publisher |
Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi |
publishDate |
2008 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9204 |
work_keys_str_mv |
AT nymokaitlin correlationbetweenfertilizationcleavageandpregnancyratewithspermdnafragmentationindexdfi |
_version_ |
1716515382089482240 |