Ground state depletion microscopy for imaging the interactions between gold nanoparticles and fluorescent molecules

• Main Author: Blythe, Karole Lynn
• Format: Others
• Language: en_US
• Published: 2013
• Subjects: Gold nanowires; Gold nanorods; Super-resolution imaging; Ground state depletion microscopy
• Online Access: http://hdl.handle.net/2152/19653

Ground state depletion with individual molecule return (GSDIM) super-resolution microscopy is used to interrogate the location of individual fluorescence bursts from two different nanoparticle-fluorophore systems. The first system consists of fluorophore-labeled DNA molecules on gold nanowire surfaces. In this system carboxytetramethyl rhodamine-labeled double-stranded DNA molecules were bound to the surface of gold nanowires via gold-thiol linkages. The second system focuses on mesoporous silica coated nanorods with dye embedded into the silica coating. The dye molecule, Rhodamine 6G, was incorporated into the silica shell during the nanorod coating procedure. Individual fluorescence bursts were spatially localized using point spread function fitting and used to reconstruct the image of the underlying nanowire or nanorod. === text