Development of Novel Ion Mobility-Mass Spectrometry Shift Reagents for Proteomic Applications

Development of Novel Ion Mobility-Mass Spectrometry Shift Reagents for Proteomic Applications

Novel ion mobility-mass spectrometry shift reagents have been developed in order to facilitate rapid identification of specific functionalities within a complex mixture of peptides. Lanthanide chelating labels provide great flexibility and can be used for relative quantitation experiments, as well a...

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Bibliographic Details
Main Author: Kerr, Thomas Joshua
Other Authors: Prof. John McLean
Format: Others
Language:en
Published: VANDERBILT 2011
Subjects:
Chemistry
Online Access:http://etd.library.vanderbilt.edu/available/etd-03282011-140349/
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spelling ndltd-VANDERBILT-oai-VANDERBILTETD-etd-03282011-1403492013-01-08T17:16:47Z Development of Novel Ion Mobility-Mass Spectrometry Shift Reagents for Proteomic Applications Kerr, Thomas Joshua Chemistry Novel ion mobility-mass spectrometry shift reagents have been developed in order to facilitate rapid identification of specific functionalities within a complex mixture of peptides. Lanthanide chelating labels provide great flexibility and can be used for relative quantitation experiments, as well as IM-MS shift reagents when analyzed using ion mobility instrumentation. Evaluation of optimum labeling strategies and limitations were explored and complex samples were analyzed for the application of these shift reagents to analysis of complex mixtures of peptides. Furthermore, progress toward developing shift reagents for use in labeling other functionalities is briefly outlined. Prof. John McLean Prof. David Cliffel Prof. Eva Harth Prof. Chris Janetopoulos VANDERBILT 2011-04-15 text application/pdf http://etd.library.vanderbilt.edu/available/etd-03282011-140349/ http://etd.library.vanderbilt.edu/available/etd-03282011-140349/ en unrestricted I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.
collection NDLTD
language en
format Others
sources NDLTD
topic Chemistry
spellingShingle Chemistry
Kerr, Thomas Joshua
Development of Novel Ion Mobility-Mass Spectrometry Shift Reagents for Proteomic Applications
description Novel ion mobility-mass spectrometry shift reagents have been developed in order to facilitate rapid identification of specific functionalities within a complex mixture of peptides. Lanthanide chelating labels provide great flexibility and can be used for relative quantitation experiments, as well as IM-MS shift reagents when analyzed using ion mobility instrumentation. Evaluation of optimum labeling strategies and limitations were explored and complex samples were analyzed for the application of these shift reagents to analysis of complex mixtures of peptides. Furthermore, progress toward developing shift reagents for use in labeling other functionalities is briefly outlined.
author2 Prof. John McLean
author_facet Prof. John McLean
Kerr, Thomas Joshua
author Kerr, Thomas Joshua
author_sort Kerr, Thomas Joshua
title Development of Novel Ion Mobility-Mass Spectrometry Shift Reagents for Proteomic Applications
title_short Development of Novel Ion Mobility-Mass Spectrometry Shift Reagents for Proteomic Applications
title_full Development of Novel Ion Mobility-Mass Spectrometry Shift Reagents for Proteomic Applications
title_fullStr Development of Novel Ion Mobility-Mass Spectrometry Shift Reagents for Proteomic Applications
title_full_unstemmed Development of Novel Ion Mobility-Mass Spectrometry Shift Reagents for Proteomic Applications
title_sort development of novel ion mobility-mass spectrometry shift reagents for proteomic applications
publisher VANDERBILT
publishDate 2011
url http://etd.library.vanderbilt.edu/available/etd-03282011-140349/
work_keys_str_mv AT kerrthomasjoshua developmentofnovelionmobilitymassspectrometryshiftreagentsforproteomicapplications
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