Bioanalytical Approaches to Chemical Biology
As the field of chemical biology evolves, so does the development of novel bioanalytical techniques capable of accurately measuring biochemical composition and regulation. This dissertation explores three aspects of chemical biology using validated analytical methodology coupled with novel nanoscale...
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ndltd-VANDERBILT-oai-VANDERBILTETD-etd-06012010-1014032013-01-08T17:16:39Z Bioanalytical Approaches to Chemical Biology Harry, Stephen Reese Chemistry As the field of chemical biology evolves, so does the development of novel bioanalytical techniques capable of accurately measuring biochemical composition and regulation. This dissertation explores three aspects of chemical biology using validated analytical methodology coupled with novel nanoscale intracellular detection probes. Using LC tandem mass spectrometry, identification and quantification of hydroxylated fatty acids associated with S. mansoni hemozoin was achieved. Additionally, the metabolic impact of 4-hydroxynonenal toxicity on macrophage activation was examined using multianalyte microphysiometry which confirmed a concentration and temporal inhibition of overall cellular metabolism. Utilizing the inherent characteristics of DNA-coated gold nanospheres, a new mRNA detection probe was developed that positively identifies tyrosinase-expressing melanoma cells. The broad scope of this work explores the use of new and emerging bioanalytical techniques and reagents that will be requisite for understanding biochemical activity at the single-cell level. David W Wright Brian O. Bachmann John A McLean Eric P Skaar VANDERBILT 2010-06-01 text application/pdf http://etd.library.vanderbilt.edu/available/etd-06012010-101403/ http://etd.library.vanderbilt.edu/available/etd-06012010-101403/ en unrestricted I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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Chemistry Harry, Stephen Reese Bioanalytical Approaches to Chemical Biology |
description |
As the field of chemical biology evolves, so does the development of novel bioanalytical techniques capable of accurately measuring biochemical composition and regulation. This dissertation explores three aspects of chemical biology using validated analytical methodology coupled with novel nanoscale intracellular detection probes. Using LC tandem mass spectrometry, identification and quantification of hydroxylated fatty acids associated with S. mansoni hemozoin was achieved. Additionally, the metabolic impact of 4-hydroxynonenal toxicity on macrophage activation was examined using multianalyte microphysiometry which confirmed a concentration and temporal inhibition of overall cellular metabolism. Utilizing the inherent characteristics of DNA-coated gold nanospheres, a new mRNA detection probe was developed that positively identifies tyrosinase-expressing melanoma cells. The broad scope of this work explores the use of new and emerging bioanalytical techniques and reagents that will be requisite for understanding biochemical activity at the single-cell level. |
author2 |
David W Wright |
author_facet |
David W Wright Harry, Stephen Reese |
author |
Harry, Stephen Reese |
author_sort |
Harry, Stephen Reese |
title |
Bioanalytical Approaches to Chemical Biology |
title_short |
Bioanalytical Approaches to Chemical Biology |
title_full |
Bioanalytical Approaches to Chemical Biology |
title_fullStr |
Bioanalytical Approaches to Chemical Biology |
title_full_unstemmed |
Bioanalytical Approaches to Chemical Biology |
title_sort |
bioanalytical approaches to chemical biology |
publisher |
VANDERBILT |
publishDate |
2010 |
url |
http://etd.library.vanderbilt.edu/available/etd-06012010-101403/ |
work_keys_str_mv |
AT harrystephenreese bioanalyticalapproachestochemicalbiology |
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1716570294078930944 |