Mask Projection Microstereolithography 3D Printing of Gelatin Methacrylate
Gelatin methacrylate (GelMA) is a ubiquitous biocompatible photopolymer used in tissue engineering and regenerative medicine due to its cost-effective synthesis, tunable mechanical properties, and cellular response. Biotechnology applications utilizing GelMA have ranged from developing cell-laden hy...
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ndltd-VTETD-oai-vtechworks.lib.vt.edu-10919-1010532021-12-22T06:03:22Z Mask Projection Microstereolithography 3D Printing of Gelatin Methacrylate Surbey, Wyatt R. Materials Science and Engineering Whittington, Abby R. Foster, Earl Johan Yu, Hang 3D printing gelatin methacrylate stereolithography biomaterials Gelatin methacrylate (GelMA) is a ubiquitous biocompatible photopolymer used in tissue engineering and regenerative medicine due to its cost-effective synthesis, tunable mechanical properties, and cellular response. Biotechnology applications utilizing GelMA have ranged from developing cell-laden hydrogel networks to cell encapsulation and additive manufacturing (3D printing). However, extrusion based 3D printing is the most common technique used with GelMA. Mask projection microstereolithography (MPµSL or µSL) is an advanced 3D printing technique that can produce geometries with high resolution, high complexity, and feature sizes unlike extrusion based printing. There are few biomaterials available for µSL applications, so 3D printing GelMA using µSL would not only add to the repertoire materials, but also demonstrate the advantages of µSL over other 3D printing techniques. A novel GelMA resin was tested with µSL to create a porous scaffold with a height and print time that has not been displayed in the literature before for a scaffold of this size. The resin consists of GelMA, deionized water, lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, photoinitiator), and 2-Hydroxy-4-methoxybenzophenone-5-sulfonic acid (sulisobenzone, UV blocker) and can be processed at room temperature. Four resins were tested (w/w %) and characterized for µSL printing: 20% GelMA 0.5% UV blocker, 20% GelMA 1.0% UV blocker, 30% GelMA 0.5% UV Blocker, and 30% GelMA 1.0% UV blocker. Swell testing, working curve, photo-rheology, photo-DSC (dynamic scanning calorimetry), 3D printing, and cell culture tests were performed and results showed that 30% GelMA 1.0% UV blocker had the best 3D print fidelity among resin compositions. Master of Science 2020-12-10T07:00:27Z 2020-12-10T07:00:27Z 2019-06-18 Thesis vt_gsexam:20699 http://hdl.handle.net/10919/101053 This item is protected by copyright and/or related rights. Some uses of this item may be deemed fair and permitted by law even without permission from the rights holder(s), or the rights holder(s) may have licensed the work for use under certain conditions. For other uses you need to obtain permission from the rights holder(s). ETD application/pdf application/pdf Virginia Tech |
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3D printing gelatin methacrylate stereolithography biomaterials |
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3D printing gelatin methacrylate stereolithography biomaterials Surbey, Wyatt R. Mask Projection Microstereolithography 3D Printing of Gelatin Methacrylate |
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Gelatin methacrylate (GelMA) is a ubiquitous biocompatible photopolymer used in tissue engineering and regenerative medicine due to its cost-effective synthesis, tunable mechanical properties, and cellular response. Biotechnology applications utilizing GelMA have ranged from developing cell-laden hydrogel networks to cell encapsulation and additive manufacturing (3D printing). However, extrusion based 3D printing is the most common technique used with GelMA. Mask projection microstereolithography (MPµSL or µSL) is an advanced 3D printing technique that can produce geometries with high resolution, high complexity, and feature sizes unlike extrusion based printing. There are few biomaterials available for µSL applications, so 3D printing GelMA using µSL would not only add to the repertoire materials, but also demonstrate the advantages of µSL over other 3D printing techniques. A novel GelMA resin was tested with µSL to create a porous scaffold with a height and print time that has not been displayed in the literature before for a scaffold of this size. The resin consists of GelMA, deionized water, lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, photoinitiator), and 2-Hydroxy-4-methoxybenzophenone-5-sulfonic acid (sulisobenzone, UV blocker) and can be processed at room temperature. Four resins were tested (w/w %) and characterized for µSL printing: 20% GelMA 0.5% UV blocker, 20% GelMA 1.0% UV blocker, 30% GelMA 0.5% UV Blocker, and 30% GelMA 1.0% UV blocker. Swell testing, working curve, photo-rheology, photo-DSC (dynamic scanning calorimetry), 3D printing, and cell culture tests were performed and results showed that 30% GelMA 1.0% UV blocker had the best 3D print fidelity among resin compositions. === Master of Science |
author2 |
Materials Science and Engineering |
author_facet |
Materials Science and Engineering Surbey, Wyatt R. |
author |
Surbey, Wyatt R. |
author_sort |
Surbey, Wyatt R. |
title |
Mask Projection Microstereolithography 3D Printing of Gelatin Methacrylate |
title_short |
Mask Projection Microstereolithography 3D Printing of Gelatin Methacrylate |
title_full |
Mask Projection Microstereolithography 3D Printing of Gelatin Methacrylate |
title_fullStr |
Mask Projection Microstereolithography 3D Printing of Gelatin Methacrylate |
title_full_unstemmed |
Mask Projection Microstereolithography 3D Printing of Gelatin Methacrylate |
title_sort |
mask projection microstereolithography 3d printing of gelatin methacrylate |
publisher |
Virginia Tech |
publishDate |
2020 |
url |
http://hdl.handle.net/10919/101053 |
work_keys_str_mv |
AT surbeywyattr maskprojectionmicrostereolithography3dprintingofgelatinmethacrylate |
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