Effect of krilium on the respiratory activities of Rhizobium trifolii and Agrobacterium tumefaciens on various substrates

• Main Author: Stafford, W. R.
• Other Authors: Biochemistry
• Format: Others
• Language: en
• Published: Virginia Tech 2014
• Subjects: LD5655.V855 1953.S723; Agrobacterium; Rhizobium
• Online Access: http://hdl.handle.net/10919/41952; http://scholar.lib.vt.edu/theses/available/etd-04072010-020017/

The respiratory rates of Rhizobium trifolii and Agrobacterium tumefaciens were measured by use of a Warburg Respirometer. A non-proliferating or "resting cell" technique was utilized in demonstrating the effect of Krilium on the oxygen consumption of the organisms when Krilium was aided to a given substrate, glucose, succinate or mannitol. Two types of Krilium were used in this investigation, Krilium (Blend #6) and Krilium (Blend #9). Observations were made on the effect of various concentrations (.05%, .10% and 1.0%) of the two blends of Krilium on the respiratory activities of the test organisms. It was observed that Krillium (Blend #6) caused a noticeable increase in the respiratory rate of Rhizobium trifolii, whereas in the case of Agrobacterium tumefaciens, no effect or a slight decrease was observed when Krilium (Blend #9) was added to the substrate, A very noticeable decreases in respiratory rates was observed when both test organisms were permitted to respire on substrates containing Krilium (Blend #9). When Krilium (Blend #6) and Krilium (Blend #9) were used as the only substrates, an increase in respiration rates of the organisms was observed. It is indicative from this work that Krilium (Blend #6) either causes a slight increase In the respiratory activities or has no deleterious effect on the respiration of the test organisms., The results obtained, when using Krilium (Blend #9), seem to indicate that this blend of Krillum has an undesirable effect on the respiration of the two organisms used in this investigation. === Master of Science