Factors affecting preservation of liquid and frozen ram spermatozoa
<p>A comparison of the results of Experiments 1 and 2 vividly demonstrates the vulnerability of ram spermatozoa to the stress of freeze-thawing. When ram spermatozoa were subjected to freeze-thaw stress, there was more variation among treatments reflected in maintenance of both intact acrosome...
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ndltd-VTETD-oai-vtechworks.lib.vt.edu-10919-437292021-05-05T05:40:15Z Factors affecting preservation of liquid and frozen ram spermatozoa Johnson, Larry Animal Science Meacham, Thomas N. Wise, Milton B. Saacke, Richard G. LD5655.V855 1974.J64 Frozen semen Semen <p>A comparison of the results of Experiments 1 and 2 vividly demonstrates the vulnerability of ram spermatozoa to the stress of freeze-thawing. When ram spermatozoa were subjected to freeze-thaw stress, there was more variation among treatments reflected in maintenance of both intact acrosomes and motile life (Experiment 2, Table 9) than when unfrozen sperm were studied (Experiment 1, Table 5). The influence of glycerol and Tris are particularly noteworthy. Though rate of thaw is not part of the surrounding media, it does control the amount of time the cells are subjected to an even more hostile environment (high salt concentrations) encountered near the melting point of ice. Therefore, the benefit of higher thaw temperatures and resulting faster thaw rates was undoubtedly due to minimizing exposure time of spermatozoa to this adverse condition. Master of Science 2014-03-14T21:40:27Z 2014-03-14T21:40:27Z 1974-07-15 2010-07-15 2010-07-15 2010-07-15 Thesis Text etd-07152010-020103 http://hdl.handle.net/10919/43729 http://scholar.lib.vt.edu/theses/available/etd-07152010-020103/ OCLC# 21882354 LD5655.V855_1974.J64.pdf In Copyright http://rightsstatements.org/vocab/InC/1.0/ 66 leaves BTD application/pdf application/pdf Virginia Tech |
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LD5655.V855 1974.J64 Frozen semen Semen |
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LD5655.V855 1974.J64 Frozen semen Semen Johnson, Larry Factors affecting preservation of liquid and frozen ram spermatozoa |
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<p>A comparison of the results of Experiments 1 and 2 vividly demonstrates
the vulnerability of ram spermatozoa to the stress of freeze-thawing. When ram spermatozoa were subjected to freeze-thaw stress,
there was more variation among treatments reflected in maintenance of
both intact acrosomes and motile life (Experiment 2, Table 9) than when
unfrozen sperm were studied (Experiment 1, Table 5). The influence of
glycerol and Tris are particularly noteworthy. Though rate of thaw is
not part of the surrounding media, it does control the amount of time
the cells are subjected to an even more hostile environment (high salt
concentrations) encountered near the melting point of ice. Therefore,
the benefit of higher thaw temperatures and resulting faster thaw rates
was undoubtedly due to minimizing exposure time of spermatozoa to this adverse condition. === Master of Science |
author2 |
Animal Science |
author_facet |
Animal Science Johnson, Larry |
author |
Johnson, Larry |
author_sort |
Johnson, Larry |
title |
Factors affecting preservation of liquid and frozen ram spermatozoa |
title_short |
Factors affecting preservation of liquid and frozen ram spermatozoa |
title_full |
Factors affecting preservation of liquid and frozen ram spermatozoa |
title_fullStr |
Factors affecting preservation of liquid and frozen ram spermatozoa |
title_full_unstemmed |
Factors affecting preservation of liquid and frozen ram spermatozoa |
title_sort |
factors affecting preservation of liquid and frozen ram spermatozoa |
publisher |
Virginia Tech |
publishDate |
2014 |
url |
http://hdl.handle.net/10919/43729 http://scholar.lib.vt.edu/theses/available/etd-07152010-020103/ |
work_keys_str_mv |
AT johnsonlarry factorsaffectingpreservationofliquidandfrozenramspermatozoa |
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