Extraction of jojoba tannins and their role in protein-tannin complex formation and digestive proteases inhibition.
Jojoba albumins and globulins were treated with jojoba tannins to study the protein-tannin complex formation. Trypsin and chymotrypsin were also treated with jojoba tannins to determine the change of enzymatic activity. The treated albumins and globulins were fractionated by gel filtration before an...
Main Author: | |
---|---|
Other Authors: | |
Language: | en |
Published: |
The University of Arizona.
1988
|
Subjects: | |
Online Access: | http://hdl.handle.net/10150/184405 |
id |
ndltd-arizona.edu-oai-arizona.openrepository.com-10150-184405 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-arizona.edu-oai-arizona.openrepository.com-10150-1844052015-10-23T04:29:40Z Extraction of jojoba tannins and their role in protein-tannin complex formation and digestive proteases inhibition. Sanchez Lucero, Manuel. Price, Ralph L. Reid, Bobby L. Berry, James W. Marchello, John A. Weber, Charles W. Jojoba. Jojoba products. Tannins. Proteins. Jojoba albumins and globulins were treated with jojoba tannins to study the protein-tannin complex formation. Trypsin and chymotrypsin were also treated with jojoba tannins to determine the change of enzymatic activity. The treated albumins and globulins were fractionated by gel filtration before and after treatment. Two albumin fractions did not appear after treatment, but a new fraction appeared with a shorter elution time. A precipitate was formed by addition of jojoba tannins to the jojoba globulins, and two globulin fractions did not appear after treatment. Trypsin was completely inhibited by 5.80 μg of tannin/ml. Chymotrypsin was completely inhibited by 36.44 μg of tannin/ml. Seven two-hour successive extractions with methanol were sufficient to obtain a jojoba meal which, when mixed at 15% in a ration with soybean, resulted in excellent growth of mice. Detannification of jojoba meal with methanol and acid methanol extracted other toxicants, because mice fed 15% of this jojoba meal gained more weight than the control. Acid methanol extraction was more effective than methanol or aqueous extraction in removing tannins; water extracted more protein and less tannin. 1988 text Dissertation-Reproduction (electronic) http://hdl.handle.net/10150/184405 701247946 8814272 en Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. The University of Arizona. |
collection |
NDLTD |
language |
en |
sources |
NDLTD |
topic |
Jojoba. Jojoba products. Tannins. Proteins. |
spellingShingle |
Jojoba. Jojoba products. Tannins. Proteins. Sanchez Lucero, Manuel. Extraction of jojoba tannins and their role in protein-tannin complex formation and digestive proteases inhibition. |
description |
Jojoba albumins and globulins were treated with jojoba tannins to study the protein-tannin complex formation. Trypsin and chymotrypsin were also treated with jojoba tannins to determine the change of enzymatic activity. The treated albumins and globulins were fractionated by gel filtration before and after treatment. Two albumin fractions did not appear after treatment, but a new fraction appeared with a shorter elution time. A precipitate was formed by addition of jojoba tannins to the jojoba globulins, and two globulin fractions did not appear after treatment. Trypsin was completely inhibited by 5.80 μg of tannin/ml. Chymotrypsin was completely inhibited by 36.44 μg of tannin/ml. Seven two-hour successive extractions with methanol were sufficient to obtain a jojoba meal which, when mixed at 15% in a ration with soybean, resulted in excellent growth of mice. Detannification of jojoba meal with methanol and acid methanol extracted other toxicants, because mice fed 15% of this jojoba meal gained more weight than the control. Acid methanol extraction was more effective than methanol or aqueous extraction in removing tannins; water extracted more protein and less tannin. |
author2 |
Price, Ralph L. |
author_facet |
Price, Ralph L. Sanchez Lucero, Manuel. |
author |
Sanchez Lucero, Manuel. |
author_sort |
Sanchez Lucero, Manuel. |
title |
Extraction of jojoba tannins and their role in protein-tannin complex formation and digestive proteases inhibition. |
title_short |
Extraction of jojoba tannins and their role in protein-tannin complex formation and digestive proteases inhibition. |
title_full |
Extraction of jojoba tannins and their role in protein-tannin complex formation and digestive proteases inhibition. |
title_fullStr |
Extraction of jojoba tannins and their role in protein-tannin complex formation and digestive proteases inhibition. |
title_full_unstemmed |
Extraction of jojoba tannins and their role in protein-tannin complex formation and digestive proteases inhibition. |
title_sort |
extraction of jojoba tannins and their role in protein-tannin complex formation and digestive proteases inhibition. |
publisher |
The University of Arizona. |
publishDate |
1988 |
url |
http://hdl.handle.net/10150/184405 |
work_keys_str_mv |
AT sanchezluceromanuel extractionofjojobatanninsandtheirroleinproteintannincomplexformationanddigestiveproteasesinhibition |
_version_ |
1718097350952484864 |