RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS

RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS

The responses of the tepary bean (Phaseolus acutifolius) to in vitro tissue culture systems were documented. Tests were conducted to identify the optimal auxin and cytokinin combinations required for optimal callus growth. Regeneration experiments were conducted to: (1) determine the effect of expla...

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Main Author: Lormand, Katherine Bradbury, 1961-
Language:en_US
Published: The University of Arizona. 1987
Subjects:
Tepary bean > Micropropagation.
Plant tissue culture.
Online Access:http://hdl.handle.net/10150/276474
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spelling ndltd-arizona.edu-oai-arizona.openrepository.com-10150-2764742015-11-21T03:00:57Z RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS Lormand, Katherine Bradbury, 1961- Tepary bean -- Micropropagation. Plant tissue culture. The responses of the tepary bean (Phaseolus acutifolius) to in vitro tissue culture systems were documented. Tests were conducted to identify the optimal auxin and cytokinin combinations required for optimal callus growth. Regeneration experiments were conducted to: (1) determine the effect of explant source and age on regeneration, (2) effect of callus age on regeneration, (3) the cultivation status of the explant source, and (4) the effects of nutritional additives on somatic embryogenesis. The callus was easily induced and maintained in all hormonal medias except those containing IAA and 6BA. The results for regeneration were most promising from cultures derived from immature cotyledon tissue. Ammonium Chloride, glutamine and Absisic acid appeared to have little affect on embryogenesis, however the addition of kinetin enabled the embryos to develop to the torpedo stage. Callus age and cultivative status of explant source had no effects on plantlet regeneration. 1987 text Thesis-Reproduction (electronic) http://hdl.handle.net/10150/276474 18826120 1331415 .b16648948 en_US Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. The University of Arizona.
collection NDLTD
language en_US
sources NDLTD
topic Tepary bean -- Micropropagation.
Plant tissue culture.
spellingShingle Tepary bean -- Micropropagation.
Plant tissue culture.
Lormand, Katherine Bradbury, 1961-
RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS
description The responses of the tepary bean (Phaseolus acutifolius) to in vitro tissue culture systems were documented. Tests were conducted to identify the optimal auxin and cytokinin combinations required for optimal callus growth. Regeneration experiments were conducted to: (1) determine the effect of explant source and age on regeneration, (2) effect of callus age on regeneration, (3) the cultivation status of the explant source, and (4) the effects of nutritional additives on somatic embryogenesis. The callus was easily induced and maintained in all hormonal medias except those containing IAA and 6BA. The results for regeneration were most promising from cultures derived from immature cotyledon tissue. Ammonium Chloride, glutamine and Absisic acid appeared to have little affect on embryogenesis, however the addition of kinetin enabled the embryos to develop to the torpedo stage. Callus age and cultivative status of explant source had no effects on plantlet regeneration.
author Lormand, Katherine Bradbury, 1961-
author_facet Lormand, Katherine Bradbury, 1961-
author_sort Lormand, Katherine Bradbury, 1961-
title RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS
title_short RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS
title_full RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS
title_fullStr RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS
title_full_unstemmed RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS
title_sort response of the tepary bean phaseolus acutifolius a. grey, to tissue culture systems
publisher The University of Arizona.
publishDate 1987
url http://hdl.handle.net/10150/276474
work_keys_str_mv AT lormandkatherinebradbury1961 responseoftheteparybeanphaseolusacutifoliusagreytotissueculturesystems
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