Production and in vitro characterization of antibody against acetaldehyde rabbit serum albumin conjugates
Acetaldehyde, the first metabolite of ethanol, has been implicated in the pathogenesis of alcoholic liver disease. In order to investigate a possible immunologic mechanism whereby acetaldehyde might exert its toxic effect acetaldehyde protein conjuates were prepared and characterized. This study dem...
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The University of Arizona.
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ndltd-arizona.edu-oai-arizona.openrepository.com-10150-2766032015-10-23T05:01:56Z Production and in vitro characterization of antibody against acetaldehyde rabbit serum albumin conjugates Lung, Chien-Cheng, 1960- Pinnas, Jacob L. Alcohol -- Physiological effect. Immunotoxicology. Acetaldehyde, the first metabolite of ethanol, has been implicated in the pathogenesis of alcoholic liver disease. In order to investigate a possible immunologic mechanism whereby acetaldehyde might exert its toxic effect acetaldehyde protein conjuates were prepared and characterized. This study demonstrates that acetaldehyde conjugated albumin can be an immunogen, can form a more negatively charged, more acidic, heterogeneous conjugate than albumin and can elicitate a specific rabbit antibody. ELISA can be used to assay antibodies produced in response to acetaldehyde albumin conjugates suggesting that chronic alcohol ingestion can lead the generation of antibodies against acetaldehyde conjugated human serum albumin. The significance of this study is that it possibly can provide a method to investigate the mechanism responsible for the sequelae of alcoholism. 1987 text Thesis-Reproduction (electronic) http://hdl.handle.net/10150/276603 19232497 1332468 .b16733174 en_US Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. The University of Arizona. |
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en_US |
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topic |
Alcohol -- Physiological effect. Immunotoxicology. |
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Alcohol -- Physiological effect. Immunotoxicology. Lung, Chien-Cheng, 1960- Production and in vitro characterization of antibody against acetaldehyde rabbit serum albumin conjugates |
description |
Acetaldehyde, the first metabolite of ethanol, has been implicated in the pathogenesis of alcoholic liver disease. In order to investigate a possible immunologic mechanism whereby acetaldehyde might exert its toxic effect acetaldehyde protein conjuates were prepared and characterized. This study demonstrates that acetaldehyde conjugated albumin can be an immunogen, can form a more negatively charged, more acidic, heterogeneous conjugate than albumin and can elicitate a specific rabbit antibody. ELISA can be used to assay antibodies produced in response to acetaldehyde albumin conjugates suggesting that chronic alcohol ingestion can lead the generation of antibodies against acetaldehyde conjugated human serum albumin. The significance of this study is that it possibly can provide a method to investigate the mechanism responsible for the sequelae of alcoholism. |
author2 |
Pinnas, Jacob L. |
author_facet |
Pinnas, Jacob L. Lung, Chien-Cheng, 1960- |
author |
Lung, Chien-Cheng, 1960- |
author_sort |
Lung, Chien-Cheng, 1960- |
title |
Production and in vitro characterization of antibody against acetaldehyde rabbit serum albumin conjugates |
title_short |
Production and in vitro characterization of antibody against acetaldehyde rabbit serum albumin conjugates |
title_full |
Production and in vitro characterization of antibody against acetaldehyde rabbit serum albumin conjugates |
title_fullStr |
Production and in vitro characterization of antibody against acetaldehyde rabbit serum albumin conjugates |
title_full_unstemmed |
Production and in vitro characterization of antibody against acetaldehyde rabbit serum albumin conjugates |
title_sort |
production and in vitro characterization of antibody against acetaldehyde rabbit serum albumin conjugates |
publisher |
The University of Arizona. |
publishDate |
1987 |
url |
http://hdl.handle.net/10150/276603 |
work_keys_str_mv |
AT lungchiencheng1960 productionandinvitrocharacterizationofantibodyagainstacetaldehyderabbitserumalbuminconjugates |
_version_ |
1718102323573555200 |