TNS Mutagenesis: Ameliorization of the Cost of the Megaplasmid in Pseudomonas Syringae

TNS Mutagenesis: Ameliorization of the Cost of the Megaplasmid in Pseudomonas Syringae

An abnormally large plasmid has been discovered to naturally exist in two strains of Pseudomonas syringae, appropriately called the "Megaplasmid" due to the genetic content of over one million base pairs of DNA. Costs have been observed with the presence of the Megaplasmid, including growt...

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Main Author: Fanger, Christopher David
Other Authors: Maltrus, David A.
Language:en
Published: The University of Arizona. 2013
Online Access:http://hdl.handle.net/10150/297540
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spelling ndltd-arizona.edu-oai-arizona.openrepository.com-10150-2975402015-10-23T05:19:47Z TNS Mutagenesis: Ameliorization of the Cost of the Megaplasmid in Pseudomonas Syringae Fanger, Christopher David Maltrus, David A. An abnormally large plasmid has been discovered to naturally exist in two strains of Pseudomonas syringae, appropriately called the "Megaplasmid" due to the genetic content of over one million base pairs of DNA. Costs have been observed with the presence of the Megaplasmid, including growth at elevated temperatures. A technique called Tn5 mutagenesis has been utilized to ameliorate the cost to the host. Results show the initial phenotype of Tn5 mutants overcame elevated temperature growth inhibition, but presence of another mutation is likely in order for the strain to grow at elevated temperatures while harboring the Megaplasmid. Further research must be done to identify genes responsible for increased fitness. 2013 text Electronic Thesis http://hdl.handle.net/10150/297540 en Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. The University of Arizona.
collection NDLTD
language en
sources NDLTD
description An abnormally large plasmid has been discovered to naturally exist in two strains of Pseudomonas syringae, appropriately called the "Megaplasmid" due to the genetic content of over one million base pairs of DNA. Costs have been observed with the presence of the Megaplasmid, including growth at elevated temperatures. A technique called Tn5 mutagenesis has been utilized to ameliorate the cost to the host. Results show the initial phenotype of Tn5 mutants overcame elevated temperature growth inhibition, but presence of another mutation is likely in order for the strain to grow at elevated temperatures while harboring the Megaplasmid. Further research must be done to identify genes responsible for increased fitness.
author2 Maltrus, David A.
author_facet Maltrus, David A.
Fanger, Christopher David
author Fanger, Christopher David
spellingShingle Fanger, Christopher David
TNS Mutagenesis: Ameliorization of the Cost of the Megaplasmid in Pseudomonas Syringae
author_sort Fanger, Christopher David
title TNS Mutagenesis: Ameliorization of the Cost of the Megaplasmid in Pseudomonas Syringae
title_short TNS Mutagenesis: Ameliorization of the Cost of the Megaplasmid in Pseudomonas Syringae
title_full TNS Mutagenesis: Ameliorization of the Cost of the Megaplasmid in Pseudomonas Syringae
title_fullStr TNS Mutagenesis: Ameliorization of the Cost of the Megaplasmid in Pseudomonas Syringae
title_full_unstemmed TNS Mutagenesis: Ameliorization of the Cost of the Megaplasmid in Pseudomonas Syringae
title_sort tns mutagenesis: ameliorization of the cost of the megaplasmid in pseudomonas syringae
publisher The University of Arizona.
publishDate 2013
url http://hdl.handle.net/10150/297540
work_keys_str_mv AT fangerchristopherdavid tnsmutagenesisameliorizationofthecostofthemegaplasmidinpseudomonassyringae
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