Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp.
White feces syndrome (WFS) is an emerging problem for penaeid shrimp farming industries in SE Asia countries, Thailand, Malaysia, Vietnam, Indonesia, China, and in India. This occurrence of this syndrome is usually first evidenced by the appearance of white fecal strings floating on surface of the s...
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ACADEMIC PRESS INC ELSEVIER SCIENCE
2016
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ndltd-arizona.edu-oai-arizona.openrepository.com-10150-6214432016-12-01T03:00:29Z Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp. Tang, Kathy F J Han, Jee Eun Aranguren, Luis Fernando White-Noble, Brenda Schmidt, Margeaux M Piamsomboon, Patharapol Risdiana, Eris Hanggono, Bambang Univ Arizona, Sch Anim & Comparat Biochem Sci, Aquaculture Pathol Lab Shrimp disease Enterocytozoon hepatopenaei EHP White feces syndrome In situ hybridization PCR White feces syndrome (WFS) is an emerging problem for penaeid shrimp farming industries in SE Asia countries, Thailand, Malaysia, Vietnam, Indonesia, China, and in India. This occurrence of this syndrome is usually first evidenced by the appearance of white fecal strings floating on surface of the shrimp ponds. The gross signs of affected shrimp include the appearance of a whitish hindgut and loose carapace, and it is associated with reduced feeding and growth retardation. To investigate the nature of the white feces syndrome, samples of white feces and shrimp hepatopancreas tissue were collected from Penaeus vannamei in affected farms in Indonesia, and these were examined histologically. Within the white feces, we found densely packed spores of the microsporidian Enterocytozoon hepatopenaei (abbreviated as EHP) and relatively fewer numbers of rod-shaped bacteria. From WFS ponds, hepatopancreas samples form 30 individual shrimp were analyzed by histology and in situ hybridization. The results showed that all of the shrimp examined were infected with EHP accompanied by septic hepatopancreatic necrosis (SHPN). Midgut epithelial cells were also infected and this increased the number of tissue types being affected by EHP. By PCR, EHP was detected in all the samples analyzed from WFS-affected ponds, but not in those sampled from healthy shrimp ponds. To determine the modes of transmission for this parasite, we performed feeding and cohabitation bioassays, the results showed that EHP can be transmitted through per os feeding of EHP-infected hepatopancreas tissue to healthy shrimp and through cohabitation ofinfected and healthy shrimp. In addition, we found the use of Fumagillin-B, an antimicrobial agent, was ineffective in either reducing or eliminating EHP in infected shrimp. 2016-10 Article Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp. 2016, 140:1-7 J. Invertebr. Pathol. 1096-0805 27530403 10.1016/j.jip.2016.08.004 http://hdl.handle.net/10150/621443 http://arizona.openrepository.com/arizona/handle/10150/621443 Journal of invertebrate pathology en © 2016 Elsevier Inc. All rights reserved. ACADEMIC PRESS INC ELSEVIER SCIENCE |
collection |
NDLTD |
language |
en |
sources |
NDLTD |
topic |
Shrimp disease Enterocytozoon hepatopenaei EHP White feces syndrome In situ hybridization PCR |
spellingShingle |
Shrimp disease Enterocytozoon hepatopenaei EHP White feces syndrome In situ hybridization PCR Tang, Kathy F J Han, Jee Eun Aranguren, Luis Fernando White-Noble, Brenda Schmidt, Margeaux M Piamsomboon, Patharapol Risdiana, Eris Hanggono, Bambang Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp. |
description |
White feces syndrome (WFS) is an emerging problem for penaeid shrimp farming industries in SE Asia countries, Thailand, Malaysia, Vietnam, Indonesia, China, and in India. This occurrence of this syndrome is usually first evidenced by the appearance of white fecal strings floating on surface of the shrimp ponds. The gross signs of affected shrimp include the appearance of a whitish hindgut and loose carapace, and it is associated with reduced feeding and growth retardation. To investigate the nature of the white feces syndrome, samples of white feces and shrimp hepatopancreas tissue were collected from Penaeus vannamei in affected farms in Indonesia, and these were examined histologically. Within the white feces, we found densely packed spores of the microsporidian Enterocytozoon hepatopenaei (abbreviated as EHP) and relatively fewer numbers of rod-shaped bacteria. From WFS ponds, hepatopancreas samples form 30 individual shrimp were analyzed by histology and in situ hybridization. The results showed that all of the shrimp examined were infected with EHP accompanied by septic hepatopancreatic necrosis (SHPN). Midgut epithelial cells were also infected and this increased the number of tissue types being affected by EHP. By PCR, EHP was detected in all the samples analyzed from WFS-affected ponds, but not in those sampled from healthy shrimp ponds. To determine the modes of transmission for this parasite, we performed feeding and cohabitation bioassays, the results showed that EHP can be transmitted through per os feeding of EHP-infected hepatopancreas tissue to healthy shrimp and through cohabitation ofinfected and healthy shrimp. In addition, we found the use of Fumagillin-B, an antimicrobial agent, was ineffective in either reducing or eliminating EHP in infected shrimp. |
author2 |
Univ Arizona, Sch Anim & Comparat Biochem Sci, Aquaculture Pathol Lab |
author_facet |
Univ Arizona, Sch Anim & Comparat Biochem Sci, Aquaculture Pathol Lab Tang, Kathy F J Han, Jee Eun Aranguren, Luis Fernando White-Noble, Brenda Schmidt, Margeaux M Piamsomboon, Patharapol Risdiana, Eris Hanggono, Bambang |
author |
Tang, Kathy F J Han, Jee Eun Aranguren, Luis Fernando White-Noble, Brenda Schmidt, Margeaux M Piamsomboon, Patharapol Risdiana, Eris Hanggono, Bambang |
author_sort |
Tang, Kathy F J |
title |
Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp. |
title_short |
Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp. |
title_full |
Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp. |
title_fullStr |
Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp. |
title_full_unstemmed |
Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp. |
title_sort |
dense populations of the microsporidian enterocytozoon hepatopenaei (ehp) in feces of penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp. |
publisher |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
publishDate |
2016 |
url |
http://hdl.handle.net/10150/621443 http://arizona.openrepository.com/arizona/handle/10150/621443 |
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