Antitumor effects and mechanisms of Ganoderma extracts and spores oil
Ganoderma lucidum is a popular herbal medicine used in China to promote health. Modern studies have disclosed that the active ingredients of Ganoderma can exhibit several effects, including antitumor effects and immunomodulation. The present study evaluated the antitumor effects of self-prepared Gan...
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ndltd-arizona.edu-oai-arizona.openrepository.com-10150-6223622017-02-04T03:00:37Z Antitumor effects and mechanisms of Ganoderma extracts and spores oil Chen, Chun Li, Peng Li, Ye Yao, Guan Xu, Jian-Hua Systems Biology Laboratory, Department of Molecular and Cellular Biology, University of Arizona Ganoderma extracts Ganoderma spores oil tumor topoisomerase I and II cell cycle cyclin D1 Ganoderma lucidum is a popular herbal medicine used in China to promote health. Modern studies have disclosed that the active ingredients of Ganoderma can exhibit several effects, including antitumor effects and immunomodulation. The present study evaluated the antitumor effects of self-prepared Ganoderma extracts and spores oil, and investigated the possible underlying mechanisms by observing the effects of the extracts and oil on topoisomerases and the cell cycle. The results showed that Ganoderma extracts and spores oil presented dose-dependent inhibitory effects on tumor cells. The half maximal inhibitory concentration (IC50) values of Ganoderma extracts on HL60, K562 and SGC-7901 cells for 24 h were 0.44, 0.39 and 0.90 mg/ml, respectively; for Ganoderma spores oil, the IC50 values were 1.13, 2.27 and 6.29 mg/ml, respectively. In the in vivo study, the inhibitory rates of Ganoderma extracts (4 g/kg/d, intragastrically) on S180 and H22 cells were 39.1 and 44.6%, respectively, and for Ganoderma spores oil (1.2 g/kg/d, intragastrically) the inhibitory rates were 30.9 and 44.9%, respectively. Ganoderma extracts and spores oil inhibited the activities of topoisomerase I and II. Ganoderma spores oil was shown block the cell cycle at the transition between the G1 and S phases and induce a marked decrease in cyclin D1 levels in K562 cells, with no significant change in cyclin E level. These results suggest that the Ganoderma extracts and spores oil possessed antitumor effects in the in vitro and in vivo studies. The antitumor mechanisms of the extracts and spores oil were associated with inhibitory effects on topoisomerase I and II activities, and for Ganoderma spores oil, the antitumor effects may also be associated with decreased cyclin D1 levels, thus inducing G1 arrest in the cell cycle. 2016-11 Article Antitumor effects and mechanisms of Ganoderma extracts and spores oil. 2016, 12 (5):3571-3578 Oncol Lett 1792-1074 27900038 10.3892/ol.2016.5059 http://hdl.handle.net/10150/622362 http://arizona.openrepository.com/arizona/handle/10150/622362 Oncology letters en Copyright © 2016, Spandidos Publications SPANDIDOS PUBL LTD |
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Ganoderma extracts Ganoderma spores oil tumor topoisomerase I and II cell cycle cyclin D1 |
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Ganoderma extracts Ganoderma spores oil tumor topoisomerase I and II cell cycle cyclin D1 Chen, Chun Li, Peng Li, Ye Yao, Guan Xu, Jian-Hua Antitumor effects and mechanisms of Ganoderma extracts and spores oil |
description |
Ganoderma lucidum is a popular herbal medicine used in China to promote health. Modern studies have disclosed that the active ingredients of Ganoderma can exhibit several effects, including antitumor effects and immunomodulation. The present study evaluated the antitumor effects of self-prepared Ganoderma extracts and spores oil, and investigated the possible underlying mechanisms by observing the effects of the extracts and oil on topoisomerases and the cell cycle. The results showed that Ganoderma extracts and spores oil presented dose-dependent inhibitory effects on tumor cells. The half maximal inhibitory concentration (IC50) values of Ganoderma extracts on HL60, K562 and SGC-7901 cells for 24 h were 0.44, 0.39 and 0.90 mg/ml, respectively; for Ganoderma spores oil, the IC50 values were 1.13, 2.27 and 6.29 mg/ml, respectively. In the in vivo study, the inhibitory rates of Ganoderma extracts (4 g/kg/d, intragastrically) on S180 and H22 cells were 39.1 and 44.6%, respectively, and for Ganoderma spores oil (1.2 g/kg/d, intragastrically) the inhibitory rates were 30.9 and 44.9%, respectively. Ganoderma extracts and spores oil inhibited the activities of topoisomerase I and II. Ganoderma spores oil was shown block the cell cycle at the transition between the G1 and S phases and induce a marked decrease in cyclin D1 levels in K562 cells, with no significant change in cyclin E level. These results suggest that the Ganoderma extracts and spores oil possessed antitumor effects in the in vitro and in vivo studies. The antitumor mechanisms of the extracts and spores oil were associated with inhibitory effects on topoisomerase I and II activities, and for Ganoderma spores oil, the antitumor effects may also be associated with decreased cyclin D1 levels, thus inducing G1 arrest in the cell cycle. |
author2 |
Systems Biology Laboratory, Department of Molecular and Cellular Biology, University of Arizona |
author_facet |
Systems Biology Laboratory, Department of Molecular and Cellular Biology, University of Arizona Chen, Chun Li, Peng Li, Ye Yao, Guan Xu, Jian-Hua |
author |
Chen, Chun Li, Peng Li, Ye Yao, Guan Xu, Jian-Hua |
author_sort |
Chen, Chun |
title |
Antitumor effects and mechanisms of Ganoderma extracts and spores oil |
title_short |
Antitumor effects and mechanisms of Ganoderma extracts and spores oil |
title_full |
Antitumor effects and mechanisms of Ganoderma extracts and spores oil |
title_fullStr |
Antitumor effects and mechanisms of Ganoderma extracts and spores oil |
title_full_unstemmed |
Antitumor effects and mechanisms of Ganoderma extracts and spores oil |
title_sort |
antitumor effects and mechanisms of ganoderma extracts and spores oil |
publisher |
SPANDIDOS PUBL LTD |
publishDate |
2016 |
url |
http://hdl.handle.net/10150/622362 http://arizona.openrepository.com/arizona/handle/10150/622362 |
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