Detecting Oligomeric Beta-Amyloid for the Diagnosis of Alzheimer's Disease
abstract: Alzheimer's disease (AD) is the leading neurodegenerative disease, affecting roughly 8% of people 65 years of age or older. There exists an imperative need to develop a non-invasive test for the earlier detection of AD. The use of biomarkers is a promising option that examines the tox...
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ndltd-asu.edu-item-177772018-06-22T03:03:48Z Detecting Oligomeric Beta-Amyloid for the Diagnosis of Alzheimer's Disease abstract: Alzheimer's disease (AD) is the leading neurodegenerative disease, affecting roughly 8% of people 65 years of age or older. There exists an imperative need to develop a non-invasive test for the earlier detection of AD. The use of biomarkers is a promising option that examines the toxic mechanisms and metabolic pathways that cause Alzheimer's disease, eventually leading to an early diagnostic method. This thesis presents the use of oligomeric beta-amyloid as a biomarker to detect Alzheimer's disease via a specialized enzyme-linked protein assay. Specifically, this paper details the optimization and development of a novel phage capture enzyme-linked immunosorbent assay (ELISA) that can detect the relative quantity of beta-amyloid oligomers in samples from a mouse model of AD. The objective of this thesis was to optimize a phage capture ELISA using the A4 single-chain variable fragment (scFv) to quantify the amount of beta-amyloid oligomers in various mice samples. A4 selectively recognizes a toxic oligomeric form of beta-amyloid. The level of A4-reactive oligomeric beta-amyloid was measured in triplicate in homogenized mouse brain tissue samples from eight transgenic (TG) and eight nontransgenic (NTG) animals aged five, nine, and thirteen months. There was a significant difference (p < 0.0005) between the five month TG and NTG mice. A decrease in beta-amyloid levels with the aging of the TG mice suggested that the beta-amyloid oligomers may be aggregating to form beta-amyloid fibrils. Conversely, the quantity of beta-amyloid increased with the aging of the NTG mice. This indicated that beta-amyloid oligomers may develop with normal aging. Dissertation/Thesis Brownlee, Taylor (Author) Sierks, Michael (Advisor) Williams, Stephanie (Committee member) Xin, Wei (Committee member) Arizona State University (Publisher) Biomedical engineering Chemical engineering eng 52 pages M.S. Chemical Engineering 2013 Masters Thesis http://hdl.handle.net/2286/R.I.17777 http://rightsstatements.org/vocab/InC/1.0/ All Rights Reserved 2013 |
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English |
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Dissertation |
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Biomedical engineering Chemical engineering |
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Biomedical engineering Chemical engineering Detecting Oligomeric Beta-Amyloid for the Diagnosis of Alzheimer's Disease |
description |
abstract: Alzheimer's disease (AD) is the leading neurodegenerative disease, affecting roughly 8% of people 65 years of age or older. There exists an imperative need to develop a non-invasive test for the earlier detection of AD. The use of biomarkers is a promising option that examines the toxic mechanisms and metabolic pathways that cause Alzheimer's disease, eventually leading to an early diagnostic method. This thesis presents the use of oligomeric beta-amyloid as a biomarker to detect Alzheimer's disease via a specialized enzyme-linked protein assay. Specifically, this paper details the optimization and development of a novel phage capture enzyme-linked immunosorbent assay (ELISA) that can detect the relative quantity of beta-amyloid oligomers in samples from a mouse model of AD. The objective of this thesis was to optimize a phage capture ELISA using the A4 single-chain variable fragment (scFv) to quantify the amount of beta-amyloid oligomers in various mice samples. A4 selectively recognizes a toxic oligomeric form of beta-amyloid. The level of A4-reactive oligomeric beta-amyloid was measured in triplicate in homogenized mouse brain tissue samples from eight transgenic (TG) and eight nontransgenic (NTG) animals aged five, nine, and thirteen months. There was a significant difference (p < 0.0005) between the five month TG and NTG mice. A decrease in beta-amyloid levels with the aging of the TG mice suggested that the beta-amyloid oligomers may be aggregating to form beta-amyloid fibrils. Conversely, the quantity of beta-amyloid increased with the aging of the NTG mice. This indicated that beta-amyloid oligomers may develop with normal aging. === Dissertation/Thesis === M.S. Chemical Engineering 2013 |
author2 |
Brownlee, Taylor (Author) |
author_facet |
Brownlee, Taylor (Author) |
title |
Detecting Oligomeric Beta-Amyloid for the Diagnosis of Alzheimer's Disease |
title_short |
Detecting Oligomeric Beta-Amyloid for the Diagnosis of Alzheimer's Disease |
title_full |
Detecting Oligomeric Beta-Amyloid for the Diagnosis of Alzheimer's Disease |
title_fullStr |
Detecting Oligomeric Beta-Amyloid for the Diagnosis of Alzheimer's Disease |
title_full_unstemmed |
Detecting Oligomeric Beta-Amyloid for the Diagnosis of Alzheimer's Disease |
title_sort |
detecting oligomeric beta-amyloid for the diagnosis of alzheimer's disease |
publishDate |
2013 |
url |
http://hdl.handle.net/2286/R.I.17777 |
_version_ |
1718700022935060480 |