The isolation and characterisation of human cytochromes P-450
The metabolism of drugs and foreign compounds is a major influence on their pharmacological and toxicological effects. The haemoprotein cyt. P-450 exists as a multi-isozyme family which functions in the metabolism of a wide variety of foreign compounds. Biochemical information concerning the structu...
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University of Aberdeen
1987
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ndltd-bl.uk-oai-ethos.bl.uk-2346372017-04-20T03:34:29ZThe isolation and characterisation of human cytochromes P-450Shaw, P. M.1987The metabolism of drugs and foreign compounds is a major influence on their pharmacological and toxicological effects. The haemoprotein cyt. P-450 exists as a multi-isozyme family which functions in the metabolism of a wide variety of foreign compounds. Biochemical information concerning the structure and catalytic function of cyt. P-450 isozymes from man is less extensive than from animal species. In this study several microsomal proteins including four cyt. P-450 isozymes, NADPH-cyt. P-450 reductase, epoxide hydrolase and other unidentified proteins were purified from adult human liver; the methodologies previously employed to isolate cyt. P-450 isozymes were improved and now allow better resolution and recovery of human cyt. P-450. Comparison of the physical and chromatographic properties of the cyt. P-450 isozymes isolated (cyt. P-450 7:3 cyt. P-4507:4 and cyt. P-4507:5) indicated that they were very similar and probably identical. N-terminal amino acid sequence and immunochemical data demonstrated that these isozymes probably belong to the steroid-inducible gene family. Further characterisation of cyt. P-4507:3 indicated that it is a major inducible isozyme in man and that it metabolises the calcium entry blocker nifedipine but not a structurally similar drug nicardipine.572Drug metabolising enzymesUniversity of Aberdeenhttp://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.234637http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU004570Electronic Thesis or Dissertation |
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572 Drug metabolising enzymes |
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572 Drug metabolising enzymes Shaw, P. M. The isolation and characterisation of human cytochromes P-450 |
description |
The metabolism of drugs and foreign compounds is a major influence on their pharmacological and toxicological effects. The haemoprotein cyt. P-450 exists as a multi-isozyme family which functions in the metabolism of a wide variety of foreign compounds. Biochemical information concerning the structure and catalytic function of cyt. P-450 isozymes from man is less extensive than from animal species. In this study several microsomal proteins including four cyt. P-450 isozymes, NADPH-cyt. P-450 reductase, epoxide hydrolase and other unidentified proteins were purified from adult human liver; the methodologies previously employed to isolate cyt. P-450 isozymes were improved and now allow better resolution and recovery of human cyt. P-450. Comparison of the physical and chromatographic properties of the cyt. P-450 isozymes isolated (cyt. P-450 7:3 cyt. P-4507:4 and cyt. P-4507:5) indicated that they were very similar and probably identical. N-terminal amino acid sequence and immunochemical data demonstrated that these isozymes probably belong to the steroid-inducible gene family. Further characterisation of cyt. P-4507:3 indicated that it is a major inducible isozyme in man and that it metabolises the calcium entry blocker nifedipine but not a structurally similar drug nicardipine. |
author |
Shaw, P. M. |
author_facet |
Shaw, P. M. |
author_sort |
Shaw, P. M. |
title |
The isolation and characterisation of human cytochromes P-450 |
title_short |
The isolation and characterisation of human cytochromes P-450 |
title_full |
The isolation and characterisation of human cytochromes P-450 |
title_fullStr |
The isolation and characterisation of human cytochromes P-450 |
title_full_unstemmed |
The isolation and characterisation of human cytochromes P-450 |
title_sort |
isolation and characterisation of human cytochromes p-450 |
publisher |
University of Aberdeen |
publishDate |
1987 |
url |
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.234637 |
work_keys_str_mv |
AT shawpm theisolationandcharacterisationofhumancytochromesp450 AT shawpm isolationandcharacterisationofhumancytochromesp450 |
_version_ |
1718441760995147776 |