Investigating the role of chemotaxis operon genes in Agrobacterium tumefaciens

Investigating the role of chemotaxis operon genes in Agrobacterium tumefaciens

A 1.7 Kb chromosomal DNA of A. tumefaciens C58C1 downstream of chemotaxis operon was sequenced completely in both directions. The comparison of this sequence with sequence databases revealed one open reading frame with strong sequence identity to MCP gene in other bacteria. The sequencing of chromos...

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Main Author: Harighi, Behrouz
Published: Durham University 2003
Subjects:
579
Microbiology
Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272544
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spelling ndltd-bl.uk-oai-ethos.bl.uk-2725442015-03-19T05:38:23ZInvestigating the role of chemotaxis operon genes in Agrobacterium tumefaciensHarighi, Behrouz2003A 1.7 Kb chromosomal DNA of A. tumefaciens C58C1 downstream of chemotaxis operon was sequenced completely in both directions. The comparison of this sequence with sequence databases revealed one open reading frame with strong sequence identity to MCP gene in other bacteria. The sequencing of chromosomal DNA of A. tumefaciens C58 confirmed that this open reading frame has similarity with cytoplasmic domain of McpA. Four mutants of A. tumefaciens C58C1 (Cl/delYl, Cl/delY2, Cl/delB and Cl/delR) were created by in-frame deletion mutagenesis in cheYl, cheY2, cheB and cheR using pKlSmobsacB. Some phenotypic properties of mutants were studied. The cheY2, cheR and cheB mutants showed impaired chemotactic capabilities in both swarming and chemotaxis assays. Deletion of cheY1 appeared to have no significant effect on chemotaxis, under the conditions studied.579MicrobiologyDurham Universityhttp://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272544http://etheses.dur.ac.uk/4083/Electronic Thesis or Dissertation
collection NDLTD
sources NDLTD
topic 579
Microbiology
spellingShingle 579
Microbiology
Harighi, Behrouz
Investigating the role of chemotaxis operon genes in Agrobacterium tumefaciens
description A 1.7 Kb chromosomal DNA of A. tumefaciens C58C1 downstream of chemotaxis operon was sequenced completely in both directions. The comparison of this sequence with sequence databases revealed one open reading frame with strong sequence identity to MCP gene in other bacteria. The sequencing of chromosomal DNA of A. tumefaciens C58 confirmed that this open reading frame has similarity with cytoplasmic domain of McpA. Four mutants of A. tumefaciens C58C1 (Cl/delYl, Cl/delY2, Cl/delB and Cl/delR) were created by in-frame deletion mutagenesis in cheYl, cheY2, cheB and cheR using pKlSmobsacB. Some phenotypic properties of mutants were studied. The cheY2, cheR and cheB mutants showed impaired chemotactic capabilities in both swarming and chemotaxis assays. Deletion of cheY1 appeared to have no significant effect on chemotaxis, under the conditions studied.
author Harighi, Behrouz
author_facet Harighi, Behrouz
author_sort Harighi, Behrouz
title Investigating the role of chemotaxis operon genes in Agrobacterium tumefaciens
title_short Investigating the role of chemotaxis operon genes in Agrobacterium tumefaciens
title_full Investigating the role of chemotaxis operon genes in Agrobacterium tumefaciens
title_fullStr Investigating the role of chemotaxis operon genes in Agrobacterium tumefaciens
title_full_unstemmed Investigating the role of chemotaxis operon genes in Agrobacterium tumefaciens
title_sort investigating the role of chemotaxis operon genes in agrobacterium tumefaciens
publisher Durham University
publishDate 2003
url http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272544
work_keys_str_mv AT harighibehrouz investigatingtheroleofchemotaxisoperongenesinagrobacteriumtumefaciens
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