Post-transcriptional control of gluconeogenic gene expression in Saccharomyces cerevisiae
This thesis examines the post-transcriptional regulation of the gluconeogenic mRNAs <I>PCK1</I> and <I>FBP1</I> in <I>Saccharomyces cerevisiae</I>. By construction of a set of chimaeric <I>PCK1-PGK1</I> reporter genes, it was possible to show that sequ...
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ndltd-bl.uk-oai-ethos.bl.uk-3010792015-03-19T07:52:21ZPost-transcriptional control of gluconeogenic gene expression in Saccharomyces cerevisiaeChacko, Alexander D.1999This thesis examines the post-transcriptional regulation of the gluconeogenic mRNAs <I>PCK1</I> and <I>FBP1</I> in <I>Saccharomyces cerevisiae</I>. By construction of a set of chimaeric <I>PCK1-PGK1</I> reporter genes, it was possible to show that sequences from the 5' end of the <I>PCK1</I> mRNA were capable of conferring post-transcriptional glucose regulation on the reporter. These regions were presumably the ultimate target of the glucose signal on the wild-type <I>PCK1</I> mRNA. Sequences from the 5' end of the <I>SDH2</I> mRNA had previously been shown to be required for the rapid degradation of that transcript observed in the presence of glucose. In addition, a number of other elements within the <I>PCK1</I> gene proved capable of constitutively destabilising the reporter mRNA. One of these bore similarity to a 70 bp mRNA destabilising element from the highly unstable <I>MATα1</I> mRNA. Overall, the decay patterns of the chimaeric <I>PCK1</I>-<I>PGK1</I> and <I>FBP1-PGK1</I> mRNAs were consistent with other studies of the decay of chimaeric <I>PGK1</I> mRNAs.572.8GeneticsUniversity of Aberdeenhttp://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301079Electronic Thesis or Dissertation |
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572.8 Genetics |
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572.8 Genetics Chacko, Alexander D. Post-transcriptional control of gluconeogenic gene expression in Saccharomyces cerevisiae |
description |
This thesis examines the post-transcriptional regulation of the gluconeogenic mRNAs <I>PCK1</I> and <I>FBP1</I> in <I>Saccharomyces cerevisiae</I>. By construction of a set of chimaeric <I>PCK1-PGK1</I> reporter genes, it was possible to show that sequences from the 5' end of the <I>PCK1</I> mRNA were capable of conferring post-transcriptional glucose regulation on the reporter. These regions were presumably the ultimate target of the glucose signal on the wild-type <I>PCK1</I> mRNA. Sequences from the 5' end of the <I>SDH2</I> mRNA had previously been shown to be required for the rapid degradation of that transcript observed in the presence of glucose. In addition, a number of other elements within the <I>PCK1</I> gene proved capable of constitutively destabilising the reporter mRNA. One of these bore similarity to a 70 bp mRNA destabilising element from the highly unstable <I>MATα1</I> mRNA. Overall, the decay patterns of the chimaeric <I>PCK1</I>-<I>PGK1</I> and <I>FBP1-PGK1</I> mRNAs were consistent with other studies of the decay of chimaeric <I>PGK1</I> mRNAs. |
author |
Chacko, Alexander D. |
author_facet |
Chacko, Alexander D. |
author_sort |
Chacko, Alexander D. |
title |
Post-transcriptional control of gluconeogenic gene expression in Saccharomyces cerevisiae |
title_short |
Post-transcriptional control of gluconeogenic gene expression in Saccharomyces cerevisiae |
title_full |
Post-transcriptional control of gluconeogenic gene expression in Saccharomyces cerevisiae |
title_fullStr |
Post-transcriptional control of gluconeogenic gene expression in Saccharomyces cerevisiae |
title_full_unstemmed |
Post-transcriptional control of gluconeogenic gene expression in Saccharomyces cerevisiae |
title_sort |
post-transcriptional control of gluconeogenic gene expression in saccharomyces cerevisiae |
publisher |
University of Aberdeen |
publishDate |
1999 |
url |
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301079 |
work_keys_str_mv |
AT chackoalexanderd posttranscriptionalcontrolofgluconeogenicgeneexpressioninsaccharomycescerevisiae |
_version_ |
1716759507139297280 |