Interactions of the fungicide 2-aminobutane with potato tubers
A synthetic route to 2-aminobutane (2-AB) was developed, which enabled the synthesis of specifically and uniformly 14C labelled 2-AB to be carried out. 14C labelling was employed as a means of detecting 2-aminobutane in tuber tissue samples. An analytical technique, based on acid extraction and scin...
Main Author: | |
---|---|
Published: |
University of Edinburgh
1987
|
Subjects: | |
Online Access: | https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373399 |
id |
ndltd-bl.uk-oai-ethos.bl.uk-373399 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-bl.uk-oai-ethos.bl.uk-3733992018-10-09T03:23:40ZInteractions of the fungicide 2-aminobutane with potato tubersMalone, James1987A synthetic route to 2-aminobutane (2-AB) was developed, which enabled the synthesis of specifically and uniformly 14C labelled 2-AB to be carried out. 14C labelling was employed as a means of detecting 2-aminobutane in tuber tissue samples. An analytical technique, based on acid extraction and scintillation counting, was developed for the determination of 2-AB residues in potato tubers. This allowed studies to be carried out on various factors influencing the efficiency of fumigation of potato tubers with 2-aminobutane. Using C 2-AB and a specially constructed small scale fumigation chamber, an investigation into the absorption and penetration of 2-AB into tubers was undertaken. The rate and extent of 2-AB absorption were studied, and also the effect of initial fumigant concentration and length of exposure period, on the final 2-AB residues in tubers. The depth of penetration was shown to be limited to the first 1 cm of tissue indicating that after initial absorption, the amine is firmly bound at or near the tuber surface. Thin layer chromatography of extracts from fumigated tubers,provided evidence which suggested that 2-AB is not metabolised by the tubers during the storage period. Damaged tubers were shown to absorb higher levels of 2-AB, and the effect of wound healing at different temperatures, prior to fumigation, was demonstrated. The preferential uptake of 2-aminobutane through tuberlenticels was highlighted as a possible cause of tuberto tuber variation in 2-AB residues. Studies showed that there was no appreciable loss of 2-AB from fumigated tubers during the storage period, and that transfer of 2-AB from tuber to tuber during air recirculation was very limited. The absorption of 2-AB by soil, straw and wood,was examined and identified as a likely sink for the amine fumigant, and this could contribute to the low 2-AB residues recorded for some commercial fumigations. A comparison of the antifungal activity of the optical isomers of 2-aminobutane, confirmed that the R-(-)- enantiomer was more active than the corresponding S-(+)- isomer, or racemic mixture, against the fungi responsible for gangrene and skin spot in potato tubers.577EcologyUniversity of Edinburghhttps://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373399http://hdl.handle.net/1842/28529Electronic Thesis or Dissertation |
collection |
NDLTD |
sources |
NDLTD |
topic |
577 Ecology |
spellingShingle |
577 Ecology Malone, James Interactions of the fungicide 2-aminobutane with potato tubers |
description |
A synthetic route to 2-aminobutane (2-AB) was developed, which enabled the synthesis of specifically and uniformly 14C labelled 2-AB to be carried out. 14C labelling was employed as a means of detecting 2-aminobutane in tuber tissue samples. An analytical technique, based on acid extraction and scintillation counting, was developed for the determination of 2-AB residues in potato tubers. This allowed studies to be carried out on various factors influencing the efficiency of fumigation of potato tubers with 2-aminobutane. Using C 2-AB and a specially constructed small scale fumigation chamber, an investigation into the absorption and penetration of 2-AB into tubers was undertaken. The rate and extent of 2-AB absorption were studied, and also the effect of initial fumigant concentration and length of exposure period, on the final 2-AB residues in tubers. The depth of penetration was shown to be limited to the first 1 cm of tissue indicating that after initial absorption, the amine is firmly bound at or near the tuber surface. Thin layer chromatography of extracts from fumigated tubers,provided evidence which suggested that 2-AB is not metabolised by the tubers during the storage period. Damaged tubers were shown to absorb higher levels of 2-AB, and the effect of wound healing at different temperatures, prior to fumigation, was demonstrated. The preferential uptake of 2-aminobutane through tuberlenticels was highlighted as a possible cause of tuberto tuber variation in 2-AB residues. Studies showed that there was no appreciable loss of 2-AB from fumigated tubers during the storage period, and that transfer of 2-AB from tuber to tuber during air recirculation was very limited. The absorption of 2-AB by soil, straw and wood,was examined and identified as a likely sink for the amine fumigant, and this could contribute to the low 2-AB residues recorded for some commercial fumigations. A comparison of the antifungal activity of the optical isomers of 2-aminobutane, confirmed that the R-(-)- enantiomer was more active than the corresponding S-(+)- isomer, or racemic mixture, against the fungi responsible for gangrene and skin spot in potato tubers. |
author |
Malone, James |
author_facet |
Malone, James |
author_sort |
Malone, James |
title |
Interactions of the fungicide 2-aminobutane with potato tubers |
title_short |
Interactions of the fungicide 2-aminobutane with potato tubers |
title_full |
Interactions of the fungicide 2-aminobutane with potato tubers |
title_fullStr |
Interactions of the fungicide 2-aminobutane with potato tubers |
title_full_unstemmed |
Interactions of the fungicide 2-aminobutane with potato tubers |
title_sort |
interactions of the fungicide 2-aminobutane with potato tubers |
publisher |
University of Edinburgh |
publishDate |
1987 |
url |
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373399 |
work_keys_str_mv |
AT malonejames interactionsofthefungicide2aminobutanewithpotatotubers |
_version_ |
1718771810454994944 |