Wax ester biosynthesis in a calanoid copepod, Calanus finmarchicus and a fresh-water teleost, Trichogaster trichopterus
The <i>de novo</i> formation of long-chain fatty acids and the reduction of long-chain fatty acids to the corresponding fatty alcohol were studied in the calanoid copepod <i>Calanus finmarchicus (Calanus</i>) and the fresh-water fish, <i>Trichogaster trichopterus</i&...
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University of Aberdeen
1987
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ndltd-bl.uk-oai-ethos.bl.uk-3798142015-03-19T07:49:42ZWax ester biosynthesis in a calanoid copepod, Calanus finmarchicus and a fresh-water teleost, Trichogaster trichopterusRussell, Y.1987The <i>de novo</i> formation of long-chain fatty acids and the reduction of long-chain fatty acids to the corresponding fatty alcohol were studied in the calanoid copepod <i>Calanus finmarchicus (Calanus</i>) and the fresh-water fish, <i>Trichogaster trichopterus</i> (the gourami). <i>De novo</i> biosynthesis of fatty acids by the enzyme fatty acid synthase (FAS) was localised in the 6.3 x 10<sup>6</sup>g-min supernatant ('cytosolic' fraction) of homogenates of whole <i>Calanus</i> and was characteristic of a Type 1 multienzyme complex. The formation of fatty acids required the presence of acetyl-CoA, malonyl-CoA and was specific for NADPH as the reductant. The main products of the reaction were the saturated long-chain fatty acids stearic acid and palmitic acid, accounting for 54% and 25% of the total fatty acid product, respectively. The alcohol-forming enzyme, NADPH-fatty acyl-CoA oxidoreductase, from both <i>Calanus</i> the gourami roe was membrane-associated. Differential ultracentrifugation showed that a 6.3 x 10<sup>6</sup>g-min pellet (the 'particulate' fraction) was most active in the formation of long-chain fatty acohols. The specific activity of the enzyme in the gourami roe particulate fraction was over 100-fold higher than that in the <i>Calanus</i> particulate fraction. Both systems required the presence of an acyl-CoA generating system in the form of exogenous CoASH, ATP and Mg<sup>++</sup>. The reaction was specific for NADPH as the reductant in the case of the gourami roe system. However, NADH substituted for NADPH in the <i>Calanus</i> system. Palmitic acid, in the presence of an acyl-CoA generating system, was the most effective substrate with the 18:0, 18:1 and 22:1 substrates giving progressively lower activities.572Biosynthesis of wax estersUniversity of Aberdeenhttp://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379814Electronic Thesis or Dissertation |
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572 Biosynthesis of wax esters |
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572 Biosynthesis of wax esters Russell, Y. Wax ester biosynthesis in a calanoid copepod, Calanus finmarchicus and a fresh-water teleost, Trichogaster trichopterus |
description |
The <i>de novo</i> formation of long-chain fatty acids and the reduction of long-chain fatty acids to the corresponding fatty alcohol were studied in the calanoid copepod <i>Calanus finmarchicus (Calanus</i>) and the fresh-water fish, <i>Trichogaster trichopterus</i> (the gourami). <i>De novo</i> biosynthesis of fatty acids by the enzyme fatty acid synthase (FAS) was localised in the 6.3 x 10<sup>6</sup>g-min supernatant ('cytosolic' fraction) of homogenates of whole <i>Calanus</i> and was characteristic of a Type 1 multienzyme complex. The formation of fatty acids required the presence of acetyl-CoA, malonyl-CoA and was specific for NADPH as the reductant. The main products of the reaction were the saturated long-chain fatty acids stearic acid and palmitic acid, accounting for 54% and 25% of the total fatty acid product, respectively. The alcohol-forming enzyme, NADPH-fatty acyl-CoA oxidoreductase, from both <i>Calanus</i> the gourami roe was membrane-associated. Differential ultracentrifugation showed that a 6.3 x 10<sup>6</sup>g-min pellet (the 'particulate' fraction) was most active in the formation of long-chain fatty acohols. The specific activity of the enzyme in the gourami roe particulate fraction was over 100-fold higher than that in the <i>Calanus</i> particulate fraction. Both systems required the presence of an acyl-CoA generating system in the form of exogenous CoASH, ATP and Mg<sup>++</sup>. The reaction was specific for NADPH as the reductant in the case of the gourami roe system. However, NADH substituted for NADPH in the <i>Calanus</i> system. Palmitic acid, in the presence of an acyl-CoA generating system, was the most effective substrate with the 18:0, 18:1 and 22:1 substrates giving progressively lower activities. |
author |
Russell, Y. |
author_facet |
Russell, Y. |
author_sort |
Russell, Y. |
title |
Wax ester biosynthesis in a calanoid copepod, Calanus finmarchicus and a fresh-water teleost, Trichogaster trichopterus |
title_short |
Wax ester biosynthesis in a calanoid copepod, Calanus finmarchicus and a fresh-water teleost, Trichogaster trichopterus |
title_full |
Wax ester biosynthesis in a calanoid copepod, Calanus finmarchicus and a fresh-water teleost, Trichogaster trichopterus |
title_fullStr |
Wax ester biosynthesis in a calanoid copepod, Calanus finmarchicus and a fresh-water teleost, Trichogaster trichopterus |
title_full_unstemmed |
Wax ester biosynthesis in a calanoid copepod, Calanus finmarchicus and a fresh-water teleost, Trichogaster trichopterus |
title_sort |
wax ester biosynthesis in a calanoid copepod, calanus finmarchicus and a fresh-water teleost, trichogaster trichopterus |
publisher |
University of Aberdeen |
publishDate |
1987 |
url |
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379814 |
work_keys_str_mv |
AT russelly waxesterbiosynthesisinacalanoidcopepodcalanusfinmarchicusandafreshwaterteleosttrichogastertrichopterus |
_version_ |
1716759238511951872 |