Radiosensitivity in bladder cancer cells

Potentially curative treatment options for patients with organ-confined transitional cell carcinoma (TCC) of the bladder (T1-4a/N0/M0) are radical cystectomy or radiotherapy (RT)-based 'bladder-preserving' regimens. A substantial number of patients who receive RT fail to respond (approxima...

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Main Author: Sherwood, Benedict T.
Published: University of Leicester 2005
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Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.416281
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spelling ndltd-bl.uk-oai-ethos.bl.uk-4162812016-12-08T03:22:14ZRadiosensitivity in bladder cancer cellsSherwood, Benedict T.2005Potentially curative treatment options for patients with organ-confined transitional cell carcinoma (TCC) of the bladder (T1-4a/N0/M0) are radical cystectomy or radiotherapy (RT)-based 'bladder-preserving' regimens. A substantial number of patients who receive RT fail to respond (approximately 50%). Consequently, a greater understanding of the mechanisms of radioresistence is required, together with predictive information regarding the response of tumours to RT. Hypoxia and intrinsic cellular Radiosensitivity (IRS) are examined here, a factors that may influence the outcome of RT.;An immunohistochemical assay using hypoxia-related carbonic anhydrase IX (CA IX) was undertaken to determine the prognostic significance of hypoxia in bladder tumours treated with RT. A modified version of the alkaline comet assay (ACA) was used to examine differences in IRS between cells derived from TCC specimens. Nuclear factors that influence comet formation (and therefore radiosensitivity) were also examined, such as DNA double strand break (DSB) rates and differences in nuclear matrix protein (NMP) composition.;CA IX immunostaining did not provide prognostic information with respect to response to radical RT. ACA analysis indicated a wide range of responses between tumours. In TCC cell lines, DSB rates are not demonstrably different in cells of differentiated radiosensitivity, however, comparative analysis of nuclear proteins identified differences in their constitutive NMPs and repair enzymes.;These results do not provide evidence that hypoxia influences outcome after RT, but support the contention that ICR is important in dictating the response of bladder tumours to RT. Furthermore, in bladder cancer cell lines of differing radiosensitivity, differences in NMP and repair enzymes are identified. Further work is required to determine whether these are of prognostic importance.616.994620642University of Leicesterhttp://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.416281http://hdl.handle.net/2381/29874Electronic Thesis or Dissertation
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sources NDLTD
topic 616.994620642
spellingShingle 616.994620642
Sherwood, Benedict T.
Radiosensitivity in bladder cancer cells
description Potentially curative treatment options for patients with organ-confined transitional cell carcinoma (TCC) of the bladder (T1-4a/N0/M0) are radical cystectomy or radiotherapy (RT)-based 'bladder-preserving' regimens. A substantial number of patients who receive RT fail to respond (approximately 50%). Consequently, a greater understanding of the mechanisms of radioresistence is required, together with predictive information regarding the response of tumours to RT. Hypoxia and intrinsic cellular Radiosensitivity (IRS) are examined here, a factors that may influence the outcome of RT.;An immunohistochemical assay using hypoxia-related carbonic anhydrase IX (CA IX) was undertaken to determine the prognostic significance of hypoxia in bladder tumours treated with RT. A modified version of the alkaline comet assay (ACA) was used to examine differences in IRS between cells derived from TCC specimens. Nuclear factors that influence comet formation (and therefore radiosensitivity) were also examined, such as DNA double strand break (DSB) rates and differences in nuclear matrix protein (NMP) composition.;CA IX immunostaining did not provide prognostic information with respect to response to radical RT. ACA analysis indicated a wide range of responses between tumours. In TCC cell lines, DSB rates are not demonstrably different in cells of differentiated radiosensitivity, however, comparative analysis of nuclear proteins identified differences in their constitutive NMPs and repair enzymes.;These results do not provide evidence that hypoxia influences outcome after RT, but support the contention that ICR is important in dictating the response of bladder tumours to RT. Furthermore, in bladder cancer cell lines of differing radiosensitivity, differences in NMP and repair enzymes are identified. Further work is required to determine whether these are of prognostic importance.
author Sherwood, Benedict T.
author_facet Sherwood, Benedict T.
author_sort Sherwood, Benedict T.
title Radiosensitivity in bladder cancer cells
title_short Radiosensitivity in bladder cancer cells
title_full Radiosensitivity in bladder cancer cells
title_fullStr Radiosensitivity in bladder cancer cells
title_full_unstemmed Radiosensitivity in bladder cancer cells
title_sort radiosensitivity in bladder cancer cells
publisher University of Leicester
publishDate 2005
url http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.416281
work_keys_str_mv AT sherwoodbenedictt radiosensitivityinbladdercancercells
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