Characterisation of fluorescently tagged human PAC1 receptors : the in vitro analysis of hPAC1-hop1 and hPAC1-bull receptor isoforms

• Main Author: Zheng, Limian
• Published: University of Strathclyde 2008
• Subjects: 572.65
• Online Access: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.502329

The pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) are neuropeptides belong to the VIP-glucagon growth hormone releasing factor-secretin superfamily. A broad spectrum of physiological effects can be elicited by PACAP and VIP via PAC₁, VPAC₁ and VPAC₂ receptors. The activation of PAC₁ receptor is PACAP-selective with relatively low affinity for VIP. The splice variants of human PACi (hPAC₁) receptor containing full N terminus and either the hopl (hPAC₁₋hop₁) or null (hPAC₁₋null) form of the third intercellular loop (ic3) had been demonstrated to mediate cyclic AMP and inositol phosphates production by PACAP38 stimulation (Lutz et aL, 2006). This present study created novel cell lines stably expressing either hPAC₁₋hop₁ or hPAC₁₋null receptors that were fused with green fluorescent protein (GFP) at the C terminus.